Abstract
Radiolytic reduction in frozen solutions and crystals is a useful method for generation of trapped intermediates in protein-based radical reactions. In this communication we define the conditions which provide the maximum yield of one electron-reduced myoglobin at 77 K using 60Co γ-irradiation in aqueous glycerol glass. The yield reached 50% after 20 kGy, was almost complete at ∼160 kGy total dose, and does not depend on the protein concentration in the range 0.01-5 mM.
Original language | English (US) |
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Pages (from-to) | 714-721 |
Number of pages | 8 |
Journal | Radiation Physics and Chemistry |
Volume | 76 |
Issue number | 4 |
DOIs | |
State | Published - Apr 2007 |
Keywords
- Cryoreduction yield
- Gamma radiolysis
- Heme protein
ASJC Scopus subject areas
- Radiation