Cryoradiolytic reduction of heme proteins: Maximizing dose-dependent yield

Ilia G. Denisov, Doreen C. Victoria, Stephen G. Sligar

Research output: Contribution to journalArticlepeer-review


Radiolytic reduction in frozen solutions and crystals is a useful method for generation of trapped intermediates in protein-based radical reactions. In this communication we define the conditions which provide the maximum yield of one electron-reduced myoglobin at 77 K using 60Co γ-irradiation in aqueous glycerol glass. The yield reached 50% after 20 kGy, was almost complete at ∼160 kGy total dose, and does not depend on the protein concentration in the range 0.01-5 mM.

Original languageEnglish (US)
Pages (from-to)714-721
Number of pages8
JournalRadiation Physics and Chemistry
Issue number4
StatePublished - Apr 2007


  • Cryoreduction yield
  • Gamma radiolysis
  • Heme protein

ASJC Scopus subject areas

  • Radiation


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