Cryoradiolytic reduction of heme proteins: Maximizing dose-dependent yield

Ilia G. Denisov; Doreen C. Victoria; Stephen G. Sligar

doi:10.1016/j.radphyschem.2006.03.002

Cryoradiolytic reduction of heme proteins: Maximizing dose-dependent yield

Ilia G. Denisov, Doreen C. Victoria, Stephen G. Sligar

Biochemistry

Research output: Contribution to journal › Article › peer-review

Abstract

Radiolytic reduction in frozen solutions and crystals is a useful method for generation of trapped intermediates in protein-based radical reactions. In this communication we define the conditions which provide the maximum yield of one electron-reduced myoglobin at 77 K using ⁶⁰Co γ-irradiation in aqueous glycerol glass. The yield reached 50% after 20 kGy, was almost complete at ∼160 kGy total dose, and does not depend on the protein concentration in the range 0.01-5 mM.

Original language	English (US)
Pages (from-to)	714-721
Number of pages	8
Journal	Radiation Physics and Chemistry
Volume	76
Issue number	4
DOIs	https://doi.org/10.1016/j.radphyschem.2006.03.002
State	Published - Apr 2007

Keywords

Cryoreduction yield
Gamma radiolysis
Heme protein

ASJC Scopus subject areas

Radiation

Online availability

10.1016/j.radphyschem.2006.03.002

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title = "Cryoradiolytic reduction of heme proteins: Maximizing dose-dependent yield",

abstract = "Radiolytic reduction in frozen solutions and crystals is a useful method for generation of trapped intermediates in protein-based radical reactions. In this communication we define the conditions which provide the maximum yield of one electron-reduced myoglobin at 77 K using 60Co γ-irradiation in aqueous glycerol glass. The yield reached 50% after 20 kGy, was almost complete at ∼160 kGy total dose, and does not depend on the protein concentration in the range 0.01-5 mM.",

keywords = "Cryoreduction yield, Gamma radiolysis, Heme protein",

author = "Denisov, {Ilia G.} and Victoria, {Doreen C.} and Sligar, {Stephen G.}",

note = "Funding Information: We gratefully acknowledge help provided by Dr. S. Toshkov (Nuclear Radiation Lab, University of Illinois, Urbana-Champaign) when using the 60 Co source and Dr. T.M. Makris for EPR spectroscopy in control experiments. This work was supported by NIH GM 33775.",

year = "2007",

month = apr,

doi = "10.1016/j.radphyschem.2006.03.002",

language = "English (US)",

volume = "76",

pages = "714--721",

journal = "Radiation Physics and Chemistry",

issn = "0969-806X",

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T1 - Cryoradiolytic reduction of heme proteins

T2 - Maximizing dose-dependent yield

AU - Denisov, Ilia G.

AU - Victoria, Doreen C.

AU - Sligar, Stephen G.

N1 - Funding Information: We gratefully acknowledge help provided by Dr. S. Toshkov (Nuclear Radiation Lab, University of Illinois, Urbana-Champaign) when using the 60 Co source and Dr. T.M. Makris for EPR spectroscopy in control experiments. This work was supported by NIH GM 33775.

PY - 2007/4

Y1 - 2007/4

N2 - Radiolytic reduction in frozen solutions and crystals is a useful method for generation of trapped intermediates in protein-based radical reactions. In this communication we define the conditions which provide the maximum yield of one electron-reduced myoglobin at 77 K using 60Co γ-irradiation in aqueous glycerol glass. The yield reached 50% after 20 kGy, was almost complete at ∼160 kGy total dose, and does not depend on the protein concentration in the range 0.01-5 mM.

AB - Radiolytic reduction in frozen solutions and crystals is a useful method for generation of trapped intermediates in protein-based radical reactions. In this communication we define the conditions which provide the maximum yield of one electron-reduced myoglobin at 77 K using 60Co γ-irradiation in aqueous glycerol glass. The yield reached 50% after 20 kGy, was almost complete at ∼160 kGy total dose, and does not depend on the protein concentration in the range 0.01-5 mM.

KW - Cryoreduction yield

KW - Gamma radiolysis

KW - Heme protein

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JO - Radiation Physics and Chemistry

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Cryoradiolytic reduction of heme proteins: Maximizing dose-dependent yield

Abstract

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