TY - JOUR
T1 - Cryopreservation of white-tailed deer (Odocoileus virginianus) semen using soybean-, liposome-, and egg yolk-based extenders
AU - Stewart, Jamie L.
AU - Shipley, Clifford F.
AU - Katich, Ashley Seder
AU - Po, Eleonora
AU - Ellerbrock, Robyn E.
AU - Lima, Fabio S.
AU - Canisso, Igor F.
N1 - Publisher Copyright:
© 2016 Elsevier B.V.
PY - 2016/8/1
Y1 - 2016/8/1
N2 - The objectives of the present study were to compare the use of soybean-based (Andromed), liposome-based (Optixcell), and egg yolk-based (Ovine Red, Triladyl, and Biladyl) extenders for cryopreservation of white-tailed deer semen. In experiment 1, ejaculates obtained from six bucks were aliquoted into the following extenders: Andromed, Ovine Red, Triladyl, and Biladyl (containing 4%, 6%, or 8% of glycerol). In experiment 2, ejaculates obtained from eight bucks were divided amongst Andromed, Ovine Red, and Optixcell extenders. Total and progressive sperm motility were assessed for each sample before and after cryopreservation using a computer-automated semen analyzer. In experiment 2, flow cytometry was used for post-thaw assessment of sperm viability (SYBR-14/PI), acrosome integrity (FITC-PNA/PI), and chromatin stability (acridine orange). In experiment 1, both Andromed and Ovine Red extenders exhibited higher post-thaw total motility than Biladyl containing 4% or 6% of glycerol (p < 0.05). Andromed also produced higher progressive motility than all other extenders (p < 0.01) before and after cryopreservation with no differences amongst the other extenders (p ≥ 0.11). In experiment 2, there were no differences in total and progressive motility between Andromed, Ovine Red, or Optixcell extenders (p ≥ 0.39). Additionally, there were no differences in sperm viability (p = 0.18), acrosome integrity in viable sperm (p ≥ 0.10), or DNA fragmentation index (p = 0.15). These results demonstrated that soybean (Andromed) and liposome-based (Optixcell) extenders are equally as effective at cryopreserving white-tailed semen as egg yolk-based Ovine Red extender, but are superior to egg yolk-based Biladyl or Triladyl extenders.
AB - The objectives of the present study were to compare the use of soybean-based (Andromed), liposome-based (Optixcell), and egg yolk-based (Ovine Red, Triladyl, and Biladyl) extenders for cryopreservation of white-tailed deer semen. In experiment 1, ejaculates obtained from six bucks were aliquoted into the following extenders: Andromed, Ovine Red, Triladyl, and Biladyl (containing 4%, 6%, or 8% of glycerol). In experiment 2, ejaculates obtained from eight bucks were divided amongst Andromed, Ovine Red, and Optixcell extenders. Total and progressive sperm motility were assessed for each sample before and after cryopreservation using a computer-automated semen analyzer. In experiment 2, flow cytometry was used for post-thaw assessment of sperm viability (SYBR-14/PI), acrosome integrity (FITC-PNA/PI), and chromatin stability (acridine orange). In experiment 1, both Andromed and Ovine Red extenders exhibited higher post-thaw total motility than Biladyl containing 4% or 6% of glycerol (p < 0.05). Andromed also produced higher progressive motility than all other extenders (p < 0.01) before and after cryopreservation with no differences amongst the other extenders (p ≥ 0.11). In experiment 2, there were no differences in total and progressive motility between Andromed, Ovine Red, or Optixcell extenders (p ≥ 0.39). Additionally, there were no differences in sperm viability (p = 0.18), acrosome integrity in viable sperm (p ≥ 0.10), or DNA fragmentation index (p = 0.15). These results demonstrated that soybean (Andromed) and liposome-based (Optixcell) extenders are equally as effective at cryopreserving white-tailed semen as egg yolk-based Ovine Red extender, but are superior to egg yolk-based Biladyl or Triladyl extenders.
KW - Cervid andrology
KW - Fluorescent probes
KW - Semen biotechnology
KW - Semen evaluation
KW - Semen freezing
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U2 - 10.1016/j.anireprosci.2016.05.006
DO - 10.1016/j.anireprosci.2016.05.006
M3 - Article
C2 - 27287191
AN - SCOPUS:84973535657
SN - 0378-4320
VL - 171
SP - 7
EP - 16
JO - Animal reproduction science
JF - Animal reproduction science
ER -