Cryogenic Storage of tomato pollen: Effect on fecundity

Erik J. Sacks, Dina A. St. Clair

Research output: Contribution to journalArticle

Abstract

The influence of cryogenic pollen storage on fruit set and seed production in tomato (Lycopersicon esculentum Mill.) was investigated. Flowers pollinated with pollen samples stored for 5 weeks at -80C, with or without 20 h precooling at 4C, had similar fruit set and number of viable seed per fruit as those pollinated with fresh pollen. Pollen samples, which were repeatedly cooled (-80C) and warmed (to 22 to 24C) for up to six cycles, continuously maintained the same viability as the fresh pollen. When cryogenically stored pollen of L. esculentum 2-837, LA359, LA3198, and LA3199 were used to pollinate LA359, the number of viable seed formed per fruit differed significantly. Results of this study suggest that pollen cryopreservation can be used successfully for tomato breeding and germplasm storage.

Original languageEnglish (US)
Pages (from-to)447-448
Number of pages2
JournalHortScience
Volume31
Issue number3
StatePublished - Jun 1 1996
Externally publishedYes

Keywords

  • Lycopersicon esculentum
  • germplasm conservation
  • low temperature
  • pollen storage
  • seed number

ASJC Scopus subject areas

  • Horticulture

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