TY - JOUR
T1 - CryoEM-based hybrid modeling approaches for structure determination
AU - Cassidy, C. Keith
AU - Himes, Benjamin A.
AU - Luthey-Schulten, Zaida
AU - Zhang, Peijun
N1 - Publisher Copyright:
© 2017 Elsevier Ltd
PY - 2018/6
Y1 - 2018/6
N2 - Recent advances in cryo-electron microscopy (cryoEM) have dramatically improved the resolutions at which vitrified biological specimens can be studied, revealing new structural and mechanistic insights over a broad range of spatial scales. Bolstered by these advances, much effort has been directed toward the development of hybrid modeling methodologies for the construction and refinement of high-fidelity atomistic models from cryoEM data. In this brief review, we will survey the key elements of cryoEM-based hybrid modeling, providing an overview of available computational tools and strategies as well as several recent applications.
AB - Recent advances in cryo-electron microscopy (cryoEM) have dramatically improved the resolutions at which vitrified biological specimens can be studied, revealing new structural and mechanistic insights over a broad range of spatial scales. Bolstered by these advances, much effort has been directed toward the development of hybrid modeling methodologies for the construction and refinement of high-fidelity atomistic models from cryoEM data. In this brief review, we will survey the key elements of cryoEM-based hybrid modeling, providing an overview of available computational tools and strategies as well as several recent applications.
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U2 - 10.1016/j.mib.2017.10.002
DO - 10.1016/j.mib.2017.10.002
M3 - Review article
C2 - 29107896
AN - SCOPUS:85032954585
SN - 1369-5274
VL - 43
SP - 14
EP - 23
JO - Current Opinion in Microbiology
JF - Current Opinion in Microbiology
ER -