Cross-validation of experimental methodologies to characterize the behavior of hydrogels

Kathleen Toohey, Sureshkumar Kalyanam, Michael Insana

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Quantitative measurements of viscoelastic material properties of soft materials such as hydrogels and tissues pose several challenges. Sample geometry (shapes, sizes and surfaces), the method of testing (compression, torsion or indentation), and the rate of loading all affect the measurements made on these materials. Additionally, hydrated media are commonly assumed to be single-phase, homogeneous materials, when in reality they are composed of a solid matrix with a fluid phase that fills the interstitial voids. In this study, spherical indentation and parallel plate shear tests are used to characterize the behavior gelatin hydrogels. The time-dependent shear moduli estimated from indentation tests (load relaxation and quasi-static) and shear stress relaxation tests are inherently different. However, the relaxed modulus estimated from all three tests agree well (within 12%) for a range of gelatin hydrogel concentrations similar in stiffness to soft tissues. Finite element analysis (FEA) using a biphasic poroviscoelastic model is used to predict the experimentally observed behavior as well as probe the solid-fluid interactions within the hydrogel. FEA is also used in parametric studies exploring the effects of geometry (height and width) of hydrogels, similar to those encountered in mechanobiology experiments of 3D cell cultures, to establish protocols that enable reliable measurements of material properties.

Original languageEnglish (US)
Title of host publicationSociety for Experimental Mechanics - SEM Annual Conference and Exposition on Experimental and Applied Mechanics 2009
Pages2597-2602
Number of pages6
Volume4
StatePublished - 2009
EventSEM Annual Conference and Exposition on Experimental and Applied Mechanics 2009 - Albuquerque, NM, United States
Duration: Jun 1 2009Jun 4 2009

Other

OtherSEM Annual Conference and Exposition on Experimental and Applied Mechanics 2009
CountryUnited States
CityAlbuquerque, NM
Period6/1/096/4/09

ASJC Scopus subject areas

  • Computational Mechanics

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  • Cite this

    Toohey, K., Kalyanam, S., & Insana, M. (2009). Cross-validation of experimental methodologies to characterize the behavior of hydrogels. In Society for Experimental Mechanics - SEM Annual Conference and Exposition on Experimental and Applied Mechanics 2009 (Vol. 4, pp. 2597-2602)