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Critical evaluation of two primers commonly used for amplification of bacterial 16S rRNA genes
Jeremy A. Frank
, Claudia I. Reich
, Shobha Sharma
, Jon S. Weisbaum
,
Brenda A. Wilson
,
Gary J. Olsen
Microbiology
Carl R. Woese Institute for Genomic Biology
College of Veterinary Medicine
Office of the Vice Chancellor for Research and Innovation
Pathobiology
School of Molecular and Cellular Biology
Biomedical and Translational Sciences
Center for Global Studies
Center for African Studies
Research output
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Article
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peer-review
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Keyphrases
Amplification Efficiency
25%
Bacterial 16S rRNA Gene
100%
DNA Samples
25%
Escherichia Coli
25%
Gardnerella Spp
25%
Gene Clone
25%
Gene number
25%
Lactobacillus Spp
25%
Linear Amplification
25%
Microbial Community
25%
Minimal Losses
25%
PCR Conditions
25%
Primer Sequence
25%
Proposed Formulation
25%
Ribosomal RNA
100%
Vaginal Samples
25%
Biochemistry, Genetics and Molecular Biology
16S Ribosomal RNA
100%
Escherichia coli
50%
Gardnerella
50%
Gene Number
50%
Lactobacillus
50%
Ribosomal RNA
100%
RNA Gene
100%
Immunology and Microbiology
Escherichia coli
33%
Gardnerella
33%
Gene Number
33%
Lactobacillus
33%
Ribosomal RNA
100%
Ribosome RNA
66%
RNA Gene
100%