CRISPR-Cas9 strategy for activation of silent Streptomyces biosynthetic gene clusters

Mingzi M. Zhang, Fong Tian Wong, Yajie Wang, Shangwen Luo, Yee Hwee Lim, Elena Heng, Wan Lin Yeo, Ryan E. Cobb, Behnam Enghiad, Ee Lui Ang, Huimin Zhao

Research output: Contribution to journalArticle

Abstract

Here we report an efficient CRISPR-Cas9 knock-in strategy to activate silent biosynthetic gene clusters (BGCs) in streptomycetes. We applied this one-step strategy to activate multiple BGCs of different classes in five Streptomyces species and triggered the production of unique metabolites, including a novel pentangular type II polyketide in Streptomyces viridochromogenes. This potentially scalable strategy complements existing activation approaches and facilitates discovery efforts to uncover new compounds with interesting bioactivities.

Original languageEnglish (US)
Pages (from-to)607-609
Number of pages3
JournalNature chemical biology
Volume13
Issue number6
DOIs
StatePublished - Jun 1 2017

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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    Zhang, M. M., Wong, F. T., Wang, Y., Luo, S., Lim, Y. H., Heng, E., Yeo, W. L., Cobb, R. E., Enghiad, B., Ang, E. L., & Zhao, H. (2017). CRISPR-Cas9 strategy for activation of silent Streptomyces biosynthetic gene clusters. Nature chemical biology, 13(6), 607-609. https://doi.org/10.1038/nchembio.2341