TY - JOUR
T1 - Creative lysins
T2 - Listeria and the engineering of antimicrobial enzymes
AU - Van Tassell, Maxwell L.
AU - Angela Daum, M.
AU - Kim, Jun Seob
AU - Miller, Michael J.
N1 - Funding Information:
This work was supported by the USDA Cooperative State Research, Education and Extension Service (Hatch project #ILLU-698-339 ). MLVT was supported by the University of Illinois Graduate College Dissertation Completion Fellowship . MAD and JSK were supported by the Energy Biosciences Institute .
Publisher Copyright:
© 2015 Elsevier Ltd.
Copyright:
Copyright 2015 Elsevier B.V., All rights reserved.
PY - 2016/2/1
Y1 - 2016/2/1
N2 - Cell wall lytic enzymes have been of increasing interest as antimicrobials for targeting Gram-positive spoilage and pathogenic bacteria, largely due to the development of strains resistant to antibiotics and bacteriophage therapy. Such lysins show considerable promise against Listeria monocytogenes, a primary concern in food-processing environments, but there is room for improvement via protein engineering. Advances in antilisterial applications could benefit from recent developments in lysin biotechnology that have largely targeted other organisms. Herein we present various considerations for the future development of lysins, including environmental factors, cell physiology concerns, and dynamics of protein architecture. Our goal is to review key developments in lysin biotechnology to provide a contextual framework for the current models of lysin-cell interactions and highlight key considerations for the characterization and design of novel lytic enzymes.
AB - Cell wall lytic enzymes have been of increasing interest as antimicrobials for targeting Gram-positive spoilage and pathogenic bacteria, largely due to the development of strains resistant to antibiotics and bacteriophage therapy. Such lysins show considerable promise against Listeria monocytogenes, a primary concern in food-processing environments, but there is room for improvement via protein engineering. Advances in antilisterial applications could benefit from recent developments in lysin biotechnology that have largely targeted other organisms. Herein we present various considerations for the future development of lysins, including environmental factors, cell physiology concerns, and dynamics of protein architecture. Our goal is to review key developments in lysin biotechnology to provide a contextual framework for the current models of lysin-cell interactions and highlight key considerations for the characterization and design of novel lytic enzymes.
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U2 - 10.1016/j.copbio.2015.10.006
DO - 10.1016/j.copbio.2015.10.006
M3 - Review article
C2 - 26710271
AN - SCOPUS:84950971603
SN - 0958-1669
VL - 37
SP - 88
EP - 96
JO - Current Opinion in Biotechnology
JF - Current Opinion in Biotechnology
ER -