Creating new specific ligand-receptor pairs for transgene regulation

Karuppiah Chockalingam, Huimin Zhao

Research output: Contribution to journalShort surveypeer-review


The creation of specifically matched ligand-receptor pairs that are orthogonal to naturally present interacting pairs is essential for the development of small molecule-regulated gene expression systems for biotechnological applications. However, for many years this task has represented a significant challenge for synthetic chemists and protein engineers. Recently, Doyle and colleagues demonstrated that highly specific ligand-receptor pairs can be engineered in a rapid fashion by creating large libraries of protein variants and applying a selection scheme to identify variants with improved activation by the target synthetic ligand.

Original languageEnglish (US)
Pages (from-to)333-335
Number of pages3
JournalTrends in Biotechnology
Issue number7
StatePublished - Jul 2005

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering


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