Creating new specific ligand-receptor pairs for transgene regulation

Karuppiah Chockalingam, Huimin Zhao

Research output: Contribution to journalShort survey

Abstract

The creation of specifically matched ligand-receptor pairs that are orthogonal to naturally present interacting pairs is essential for the development of small molecule-regulated gene expression systems for biotechnological applications. However, for many years this task has represented a significant challenge for synthetic chemists and protein engineers. Recently, Doyle and colleagues demonstrated that highly specific ligand-receptor pairs can be engineered in a rapid fashion by creating large libraries of protein variants and applying a selection scheme to identify variants with improved activation by the target synthetic ligand.

Original languageEnglish (US)
Pages (from-to)333-335
Number of pages3
JournalTrends in Biotechnology
Volume23
Issue number7
DOIs
StatePublished - Jul 1 2005

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Transgenes
Ligands
Proteins
Gene expression
Chemical activation
Gene Expression
Engineers
Molecules

ASJC Scopus subject areas

  • Bioengineering

Cite this

Creating new specific ligand-receptor pairs for transgene regulation. / Chockalingam, Karuppiah; Zhao, Huimin.

In: Trends in Biotechnology, Vol. 23, No. 7, 01.07.2005, p. 333-335.

Research output: Contribution to journalShort survey

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