Correction of a defect in mammalian GPI anchor biosynthesis by a transfected yeast gene

R. DeGasperi, L. J. Thomas, E. Sugiyama, H. M. Chang, P. J. Beck, P. Orlean, C. Albright, G. Waneck, J. F. Sambrook, C. D. Warren, E. T.H. Yeh

Research output: Contribution to journalArticlepeer-review

Abstract

Glycosylphosphatidylinositol (GPI) serves as a membrane anchor for a large number of eukaryotic proteins. A genetic approach was used to investigate the biosynthesis of GPI anchor precursors in mammalian cells. T cell hybridoma mutants that cannot synthesize dolichol-phosphate-mannose (Dol-P-Man) also do not express on their surface GPI-anchored proteins such as Thy-1 and Ly-6A. These mutants cannot form mannose-containing GPI precursors. Transfection with the yeast Dol-P-Man synthase gene rescues the synthesis of both Dol-P-Man and mannose-containing GPI precursors, as well as the surface expression of Thy-1 and Ly-6A, suggesting that Dol-P-Man is the donor of at least one mannose residue in the GPI core.

Original languageEnglish (US)
Pages (from-to)988-991
Number of pages4
JournalScience
Volume250
Issue number4983
DOIs
StatePublished - 1990
Externally publishedYes

ASJC Scopus subject areas

  • General

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