Correction of a defect in mammalian GPI anchor biosynthesis by a transfected yeast gene

R. DeGasperi; L. J. Thomas; E. Sugiyama; H. M. Chang; P. J. Beck; P. Orlean; C. Albright; G. Waneck; J. F. Sambrook; C. D. Warren; E. T.H. Yeh

doi:10.1126/science.1978413

Correction of a defect in mammalian GPI anchor biosynthesis by a transfected yeast gene

R. DeGasperi, L. J. Thomas, E. Sugiyama, H. M. Chang, P. J. Beck, P. Orlean, C. Albright, G. Waneck, J. F. Sambrook, C. D. Warren, E. T.H. Yeh

Research output: Contribution to journal › Article › peer-review

Abstract

Glycosylphosphatidylinositol (GPI) serves as a membrane anchor for a large number of eukaryotic proteins. A genetic approach was used to investigate the biosynthesis of GPI anchor precursors in mammalian cells. T cell hybridoma mutants that cannot synthesize dolichol-phosphate-mannose (Dol-P-Man) also do not express on their surface GPI-anchored proteins such as Thy-1 and Ly-6A. These mutants cannot form mannose-containing GPI precursors. Transfection with the yeast Dol-P-Man synthase gene rescues the synthesis of both Dol-P-Man and mannose-containing GPI precursors, as well as the surface expression of Thy-1 and Ly-6A, suggesting that Dol-P-Man is the donor of at least one mannose residue in the GPI core.

Original language	English (US)
Pages (from-to)	988-991
Number of pages	4
Journal	Science
Volume	250
Issue number	4983
DOIs	https://doi.org/10.1126/science.1978413
State	Published - 1990
Externally published	Yes

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title = "Correction of a defect in mammalian GPI anchor biosynthesis by a transfected yeast gene",

abstract = "Glycosylphosphatidylinositol (GPI) serves as a membrane anchor for a large number of eukaryotic proteins. A genetic approach was used to investigate the biosynthesis of GPI anchor precursors in mammalian cells. T cell hybridoma mutants that cannot synthesize dolichol-phosphate-mannose (Dol-P-Man) also do not express on their surface GPI-anchored proteins such as Thy-1 and Ly-6A. These mutants cannot form mannose-containing GPI precursors. Transfection with the yeast Dol-P-Man synthase gene rescues the synthesis of both Dol-P-Man and mannose-containing GPI precursors, as well as the surface expression of Thy-1 and Ly-6A, suggesting that Dol-P-Man is the donor of at least one mannose residue in the GPI core.",

author = "R. DeGasperi and Thomas, {L. J.} and E. Sugiyama and Chang, {H. M.} and Beck, {P. J.} and P. Orlean and C. Albright and G. Waneck and Sambrook, {J. F.} and Warren, {C. D.} and Yeh, {E. T.H.}",

year = "1990",

doi = "10.1126/science.1978413",

language = "English (US)",

volume = "250",

pages = "988--991",

journal = "Science",

issn = "0036-8075",

publisher = "American Association for the Advancement of Science",

number = "4983",

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TY - JOUR

T1 - Correction of a defect in mammalian GPI anchor biosynthesis by a transfected yeast gene

AU - DeGasperi, R.

AU - Thomas, L. J.

AU - Sugiyama, E.

AU - Chang, H. M.

AU - Beck, P. J.

AU - Orlean, P.

AU - Albright, C.

AU - Waneck, G.

AU - Sambrook, J. F.

AU - Warren, C. D.

AU - Yeh, E. T.H.

PY - 1990

Y1 - 1990

N2 - Glycosylphosphatidylinositol (GPI) serves as a membrane anchor for a large number of eukaryotic proteins. A genetic approach was used to investigate the biosynthesis of GPI anchor precursors in mammalian cells. T cell hybridoma mutants that cannot synthesize dolichol-phosphate-mannose (Dol-P-Man) also do not express on their surface GPI-anchored proteins such as Thy-1 and Ly-6A. These mutants cannot form mannose-containing GPI precursors. Transfection with the yeast Dol-P-Man synthase gene rescues the synthesis of both Dol-P-Man and mannose-containing GPI precursors, as well as the surface expression of Thy-1 and Ly-6A, suggesting that Dol-P-Man is the donor of at least one mannose residue in the GPI core.

AB - Glycosylphosphatidylinositol (GPI) serves as a membrane anchor for a large number of eukaryotic proteins. A genetic approach was used to investigate the biosynthesis of GPI anchor precursors in mammalian cells. T cell hybridoma mutants that cannot synthesize dolichol-phosphate-mannose (Dol-P-Man) also do not express on their surface GPI-anchored proteins such as Thy-1 and Ly-6A. These mutants cannot form mannose-containing GPI precursors. Transfection with the yeast Dol-P-Man synthase gene rescues the synthesis of both Dol-P-Man and mannose-containing GPI precursors, as well as the surface expression of Thy-1 and Ly-6A, suggesting that Dol-P-Man is the donor of at least one mannose residue in the GPI core.

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Correction of a defect in mammalian GPI anchor biosynthesis by a transfected yeast gene

Abstract

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