Copper oxide nanoparticle effects on root growth and hydraulic conductivity of two vegetable crops

Andrew J Margenot, Devin A. Rippner, Matt R. Dumlao, Sareh Nezami, Peter G. Green, Sanjai J. Parikh, Andrew J. McElrone

Research output: Contribution to journalArticle

Abstract

Aims: Root growth and water transport were evaluated for two vegetable crops of contrasting root architecture (lettuce, carrot) exposed to copper oxide nanoparticles (CuO NPs). Methods: 10-day seedling root growth assays were evaluated for 16 nanometer (nm) diameter CuO NP and CuCl2 control (0.8 – 798.9 mg Cu L-1). In a separate experiment, hydraulic conductivity (Kh) of root systems not previously exposed to NP was tested using 16 and 45 nm CuO NP (798.9 mg Cu L-1) relative to CuO NP-free controls, and xylem sap was assessed by TEM-EDS for presence of CuO NPs. Results: 16 nm CuO NP produced dose-dependent increases in root diameter for lettuce (+52%) and carrot (+26%) seedlings, whereas CuCl2 did not affect (lettuce) or marginally increased (carrot) root diameter. Root Kh was similarly reduced by 16 and 45 nm CuO NPs for lettuce (-46%) but not for carrot, and no Cu was identified by TEM-EDS in xylem sap. Conclusions: Adverse effects of CuO NPs on root physiology and function in the early stages of growth of two key food crops are not necessarily due to Cu2+ toxicity and can be specific to crop species. In addition to triggering root thickening, reduction of root Kh signifies that CuO NPs can compromise root water transport and thus crop performance.

Original languageEnglish (US)
Pages (from-to)333-345
Number of pages13
JournalPlant and Soil
Volume431
Issue number1-2
DOIs
StatePublished - Oct 1 2018

Fingerprint

nanoparticles
vegetable crops
hydraulic conductivity
vegetable
oxides
root growth
copper
oxide
crop
carrots
lettuce
sap
xylem
transmission electron microscopy
seedling
root architecture
crop performance
seedlings
nanoparticle
effect

Keywords

  • Carrot
  • Copper oxide
  • Hydraulic conductivity
  • Lettuce
  • Nanoparticles
  • Roots

ASJC Scopus subject areas

  • Soil Science
  • Plant Science

Cite this

Margenot, A. J., Rippner, D. A., Dumlao, M. R., Nezami, S., Green, P. G., Parikh, S. J., & McElrone, A. J. (2018). Copper oxide nanoparticle effects on root growth and hydraulic conductivity of two vegetable crops. Plant and Soil, 431(1-2), 333-345. https://doi.org/10.1007/s11104-018-3741-3

Copper oxide nanoparticle effects on root growth and hydraulic conductivity of two vegetable crops. / Margenot, Andrew J; Rippner, Devin A.; Dumlao, Matt R.; Nezami, Sareh; Green, Peter G.; Parikh, Sanjai J.; McElrone, Andrew J.

In: Plant and Soil, Vol. 431, No. 1-2, 01.10.2018, p. 333-345.

Research output: Contribution to journalArticle

Margenot, AJ, Rippner, DA, Dumlao, MR, Nezami, S, Green, PG, Parikh, SJ & McElrone, AJ 2018, 'Copper oxide nanoparticle effects on root growth and hydraulic conductivity of two vegetable crops', Plant and Soil, vol. 431, no. 1-2, pp. 333-345. https://doi.org/10.1007/s11104-018-3741-3
Margenot, Andrew J ; Rippner, Devin A. ; Dumlao, Matt R. ; Nezami, Sareh ; Green, Peter G. ; Parikh, Sanjai J. ; McElrone, Andrew J. / Copper oxide nanoparticle effects on root growth and hydraulic conductivity of two vegetable crops. In: Plant and Soil. 2018 ; Vol. 431, No. 1-2. pp. 333-345.
@article{88e4ad617782484b98900833e6b5990e,
title = "Copper oxide nanoparticle effects on root growth and hydraulic conductivity of two vegetable crops",
abstract = "Aims: Root growth and water transport were evaluated for two vegetable crops of contrasting root architecture (lettuce, carrot) exposed to copper oxide nanoparticles (CuO NPs). Methods: 10-day seedling root growth assays were evaluated for 16 nanometer (nm) diameter CuO NP and CuCl2 control (0.8 – 798.9 mg Cu L-1). In a separate experiment, hydraulic conductivity (Kh) of root systems not previously exposed to NP was tested using 16 and 45 nm CuO NP (798.9 mg Cu L-1) relative to CuO NP-free controls, and xylem sap was assessed by TEM-EDS for presence of CuO NPs. Results: 16 nm CuO NP produced dose-dependent increases in root diameter for lettuce (+52{\%}) and carrot (+26{\%}) seedlings, whereas CuCl2 did not affect (lettuce) or marginally increased (carrot) root diameter. Root Kh was similarly reduced by 16 and 45 nm CuO NPs for lettuce (-46{\%}) but not for carrot, and no Cu was identified by TEM-EDS in xylem sap. Conclusions: Adverse effects of CuO NPs on root physiology and function in the early stages of growth of two key food crops are not necessarily due to Cu2+ toxicity and can be specific to crop species. In addition to triggering root thickening, reduction of root Kh signifies that CuO NPs can compromise root water transport and thus crop performance.",
keywords = "Carrot, Copper oxide, Hydraulic conductivity, Lettuce, Nanoparticles, Roots",
author = "Margenot, {Andrew J} and Rippner, {Devin A.} and Dumlao, {Matt R.} and Sareh Nezami and Green, {Peter G.} and Parikh, {Sanjai J.} and McElrone, {Andrew J.}",
year = "2018",
month = "10",
day = "1",
doi = "10.1007/s11104-018-3741-3",
language = "English (US)",
volume = "431",
pages = "333--345",
journal = "Plant and Soil",
issn = "0032-079X",
publisher = "Springer Netherlands",
number = "1-2",

}

TY - JOUR

T1 - Copper oxide nanoparticle effects on root growth and hydraulic conductivity of two vegetable crops

AU - Margenot, Andrew J

AU - Rippner, Devin A.

AU - Dumlao, Matt R.

AU - Nezami, Sareh

AU - Green, Peter G.

AU - Parikh, Sanjai J.

AU - McElrone, Andrew J.

PY - 2018/10/1

Y1 - 2018/10/1

N2 - Aims: Root growth and water transport were evaluated for two vegetable crops of contrasting root architecture (lettuce, carrot) exposed to copper oxide nanoparticles (CuO NPs). Methods: 10-day seedling root growth assays were evaluated for 16 nanometer (nm) diameter CuO NP and CuCl2 control (0.8 – 798.9 mg Cu L-1). In a separate experiment, hydraulic conductivity (Kh) of root systems not previously exposed to NP was tested using 16 and 45 nm CuO NP (798.9 mg Cu L-1) relative to CuO NP-free controls, and xylem sap was assessed by TEM-EDS for presence of CuO NPs. Results: 16 nm CuO NP produced dose-dependent increases in root diameter for lettuce (+52%) and carrot (+26%) seedlings, whereas CuCl2 did not affect (lettuce) or marginally increased (carrot) root diameter. Root Kh was similarly reduced by 16 and 45 nm CuO NPs for lettuce (-46%) but not for carrot, and no Cu was identified by TEM-EDS in xylem sap. Conclusions: Adverse effects of CuO NPs on root physiology and function in the early stages of growth of two key food crops are not necessarily due to Cu2+ toxicity and can be specific to crop species. In addition to triggering root thickening, reduction of root Kh signifies that CuO NPs can compromise root water transport and thus crop performance.

AB - Aims: Root growth and water transport were evaluated for two vegetable crops of contrasting root architecture (lettuce, carrot) exposed to copper oxide nanoparticles (CuO NPs). Methods: 10-day seedling root growth assays were evaluated for 16 nanometer (nm) diameter CuO NP and CuCl2 control (0.8 – 798.9 mg Cu L-1). In a separate experiment, hydraulic conductivity (Kh) of root systems not previously exposed to NP was tested using 16 and 45 nm CuO NP (798.9 mg Cu L-1) relative to CuO NP-free controls, and xylem sap was assessed by TEM-EDS for presence of CuO NPs. Results: 16 nm CuO NP produced dose-dependent increases in root diameter for lettuce (+52%) and carrot (+26%) seedlings, whereas CuCl2 did not affect (lettuce) or marginally increased (carrot) root diameter. Root Kh was similarly reduced by 16 and 45 nm CuO NPs for lettuce (-46%) but not for carrot, and no Cu was identified by TEM-EDS in xylem sap. Conclusions: Adverse effects of CuO NPs on root physiology and function in the early stages of growth of two key food crops are not necessarily due to Cu2+ toxicity and can be specific to crop species. In addition to triggering root thickening, reduction of root Kh signifies that CuO NPs can compromise root water transport and thus crop performance.

KW - Carrot

KW - Copper oxide

KW - Hydraulic conductivity

KW - Lettuce

KW - Nanoparticles

KW - Roots

UR - http://www.scopus.com/inward/record.url?scp=85051677851&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85051677851&partnerID=8YFLogxK

U2 - 10.1007/s11104-018-3741-3

DO - 10.1007/s11104-018-3741-3

M3 - Article

AN - SCOPUS:85051677851

VL - 431

SP - 333

EP - 345

JO - Plant and Soil

JF - Plant and Soil

SN - 0032-079X

IS - 1-2

ER -