Coordinate expression of cytokeratins 7 and 14, vimentin, and Bcl-2 in canine cutaneous epithelial tumors and cysts

Jason Bradley Pieper, Adam Wayne Stern, Suzette M. LeClerc, Karen L. Campbell

Research output: Contribution to journalArticle

Abstract

Forty-seven canine cutaneous epithelial tumors and cysts were examined to determine coordinate expression of cytokeratins 7 (CK7) and 14 (CK14), vimentin, and Bcl-2 using commercially available antibodies. Within non-affected normal skin adjacent to tumors or cysts, CK7 expression was observed in luminal cells in apocrine glands; CK14 expression was observed in the stratum basale, stratum spinosum, stratum granulosum, basal layer of outer root sheath, sebaceous glands, and myoepithelial cells of apocrine glands; vimentin expression was observed in dermal papilla and scattered non-epithelial cells within the epidermis; and Bcl-2 expression was observed in scattered non-epithelial cells in the epidermis and some apocrine glands. The pattern of expression of CK7 and CK14 in cases of adenocarcinoma of the apocrine gland of the anal sac (CK7+/CK14–) and hepatoid gland tumors (CK7–/CK14+) may prove useful for diagnostic purposes. Loss of expression of CK14 and vimentin, identifying myoepithelial cells, was observed in apocrine and ceruminous adenocarcinomas. Differences in patterns of expression of Bcl-2 were observed between infundibular keratinizing acanthomas compared to trichoepitheliomas.

Original languageEnglish (US)
Pages (from-to)497-503
Number of pages7
JournalJournal of Veterinary Diagnostic Investigation
Volume27
Issue number4
DOIs
StatePublished - Jul 4 2015

Fingerprint

Keratin-14
Keratin-7
Apocrine Glands
vimentin
Vimentin
Canidae
Cysts
Skin
neoplasms
dogs
epidermis (animal)
Neoplasms
adenocarcinoma
Epidermis
cells
Anal Sacs
Adenocarcinoma
Acanthoma
anal glands
keratinization

Keywords

  • Bcl-2
  • canine
  • cytokeratin 14
  • cytokeratin 7
  • immunohistochemistry
  • skin
  • tumor
  • vimentin

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Coordinate expression of cytokeratins 7 and 14, vimentin, and Bcl-2 in canine cutaneous epithelial tumors and cysts. / Pieper, Jason Bradley; Stern, Adam Wayne; LeClerc, Suzette M.; Campbell, Karen L.

In: Journal of Veterinary Diagnostic Investigation, Vol. 27, No. 4, 04.07.2015, p. 497-503.

Research output: Contribution to journalArticle

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