Cooperative polymerization of α-helices induced by macromolecular architecture

Ryan Baumgartner, Hailin Fu, Ziyuan Song, Yao Lin, Jianjun Cheng

Research output: Contribution to journalArticlepeer-review

Abstract

Catalysis observed in enzymatic processes and protein polymerizations often relies on the use of supramolecular interactions and the organization of functional elements in order to gain control over the spatial and temporal elements of fundamental cellular processes. Harnessing these cooperative interactions to catalyse reactions in synthetic systems, however, remains challenging due to the difficulty in creating structurally controlled macromolecules. Here, we report a polypeptide-based macromolecule with spatially organized α-helices that can catalyse its own formation. The system consists of a linear polymeric scaffold containing a high density of initiating groups from which polypeptides are grown, forming a brush polymer. The folding of polypeptide side chains into α-helices dramatically enhances the polymerization rate due to cooperative interactions of macrodipoles between neighbouring α-helices. The parameters that affect the rate are elucidated by a two-stage kinetic model using principles from nucleation-controlled protein polymerizations; the key difference being the irreversible nature of this polymerization.

Original languageEnglish (US)
Pages (from-to)614-622
Number of pages9
JournalNature Chemistry
Volume9
Issue number7
DOIs
StatePublished - Jul 1 2017

ASJC Scopus subject areas

  • Chemistry(all)
  • Chemical Engineering(all)

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