Conversion of xylose to ethanol by recombinant Saccharomyces cerevisiae containing genes for xylose reductase and xylitol dehydrogenase from Pichia stipitis

Y. S. Jin, T. H. Lee, Y. D. Choi, Y. W. Ryu, J. H. Seo

Research output: Contribution to journalArticlepeer-review

Abstract

A recombinant Saccharomyces cerevisiae, transformed with the genes encoding xylose reductase (XYL1) and xylitol dehydrogenase (XYL2) originated from Pichia stipitis CBS 5776, was developed to directly convert xylose to ethanol. A fed-batch fermentation with the recombinant yeast produced 8.7 g ethanol/l with a yield of 0.13g ethanol/g xylose consumed.

Original languageEnglish (US)
Pages (from-to)564-567
Number of pages4
JournalJournal of Microbiology and Biotechnology
Volume10
Issue number4
StatePublished - 2000
Externally publishedYes

Keywords

  • Ethanol
  • Saccharomyces cerevisiae
  • Xylose
  • Xylose dehydrogenase
  • Xylose reductase

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology
  • Microbiology

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