TY - JOUR
T1 - Controls on Iron Reduction and Biomineralization over Broad Environmental Conditions as Suggested by the Firmicutes Orenia metallireducens Strain Z6
AU - Dong, Yiran
AU - Sanford, Robert A.
AU - Boyanov, Maxim I.
AU - Flynn, Theodore M.
AU - O'Loughlin, Edward J.
AU - Kemner, Kenneth M.
AU - George, Samantha
AU - Fouke, Kaitlyn E.
AU - Li, Shuyi
AU - Huang, Dongmei
AU - Li, Shuzhen
AU - Fouke, Bruce W.
N1 - This project was funded by National Aeronautics and Space Administration (NASA) through the NASA Astrobiology Institute under Cooperative Agreement No. NNA13AA91A issued through the Science Mission Directorate. Y.D., S.L., D.H., and S.L. were partially supported by National Natural Science Foundation of China under the contracts 41877321 and 91851211. The XAFS data collection and analyses and effort of K.M.K., M.I.B., E.J.O., and T.M.F. were supported by the Argonne Wetlands Hydrobiogeochemistry Scientific Focus Area (SFA) at Argonne National Laboratory funded by the Subsurface Biogeochemical Research Program, Office of Biological and Environmental Research, Office of Science, U.S. Department of Energy (DOE), under contract DE-AC02-06CH11357. MRCAT/EnviroCAT operations are supported by DOE and the MRCAT/EnviroCAT member institutions. This research used resources of the Advanced Photon Source, a U.S. DOE Office of Science User Facility operated for the DOE Office of Science by Argonne National Laboratory under Contract No. DE-AC02-06CH11357. We thank Mauro Sardela and Waclaw Swiech with Frederick Seitz Materials Research Laboratory Central Facilities, UIUC, for technical support for XRD and TEM. We thank Bhoopesh Mishra, Drew Latta, and the MRCAT/EnviroCAT beamline staff for assistance during XAFS data collection. We are also grateful to Juan Liu (Peking University), Chongxuan Liu (Southern University of Science and Technology), Yuanyuan Liu (Nanjing University), Mengqiang Zhu (University of Wyoming), and Zimeng Wang (Fudan University) for insightful discussion and Boyou Wang, Zhixian Liu, Weiguo Fan (China University of Geosciences (Wuhan)) for the technical support of mineral and chemical analyses.
PY - 2020/8/18
Y1 - 2020/8/18
N2 - Microbial iron reduction is a ubiquitous biogeochemical process driven by diverse microorganisms in a variety of environments. However, it is often difficult to separate the biological from the geochemical controls on bioreduction of Fe(III) oxides. Here, we investigated the primary driving factor(s) that mediate secondary iron mineral formation over a broad range of environmental conditions using a single dissimilatory iron reducer, Orenia metallireducens strain Z6. A total of 17 distinct geochemical conditions were tested with differing pH (6.5-8.5), temperature (22-50C), salinity (2-20% NaCl), anions (phosphate and sulfate), electron shuttle (anthraquinone-2,6-disulfonate), and Fe(III) oxide mineralogy (ferrihydrite, lepidocrocite, goethite, hematite, and magnetite). The observed rates and extent of iron reduction differed significantly with kint between 0.186 and 1.702 mmol L-1 day-1 and Fe(II) production ranging from 6.3% to 83.7% of the initial Fe(III). Using X-ray absorption and scattering techniques (EXAFS and XRD), we identified and assessed the relationship between secondary minerals and the specific environmental conditions. It was inferred that the observed bifurcation of the mineralization pathways may be mediated by differing extents of Fe(II) sorption on the remaining Fe(III) minerals. These results expand our understanding of the controls on biomineralization during microbial iron reduction and aid the development of practical applications.
AB - Microbial iron reduction is a ubiquitous biogeochemical process driven by diverse microorganisms in a variety of environments. However, it is often difficult to separate the biological from the geochemical controls on bioreduction of Fe(III) oxides. Here, we investigated the primary driving factor(s) that mediate secondary iron mineral formation over a broad range of environmental conditions using a single dissimilatory iron reducer, Orenia metallireducens strain Z6. A total of 17 distinct geochemical conditions were tested with differing pH (6.5-8.5), temperature (22-50C), salinity (2-20% NaCl), anions (phosphate and sulfate), electron shuttle (anthraquinone-2,6-disulfonate), and Fe(III) oxide mineralogy (ferrihydrite, lepidocrocite, goethite, hematite, and magnetite). The observed rates and extent of iron reduction differed significantly with kint between 0.186 and 1.702 mmol L-1 day-1 and Fe(II) production ranging from 6.3% to 83.7% of the initial Fe(III). Using X-ray absorption and scattering techniques (EXAFS and XRD), we identified and assessed the relationship between secondary minerals and the specific environmental conditions. It was inferred that the observed bifurcation of the mineralization pathways may be mediated by differing extents of Fe(II) sorption on the remaining Fe(III) minerals. These results expand our understanding of the controls on biomineralization during microbial iron reduction and aid the development of practical applications.
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U2 - 10.1021/acs.est.0c03853
DO - 10.1021/acs.est.0c03853
M3 - Article
C2 - 32693580
AN - SCOPUS:85089710020
SN - 0013-936X
VL - 54
SP - 10128
EP - 10140
JO - Environmental Science and Technology
JF - Environmental Science and Technology
IS - 16
ER -