Abstract
Our previous efforts have used in vitro selection to identify numerous Zn2+-dependent deoxyribozymes that ligate two RNA substrates with reaction at a 2′,3′-cyclic phosphate. Each deoxyribozyme creates one of several different RNA linkages, including native 3′-5′ and non-native 2′-5′ phosphodiester bonds as well as many unnatural linkages. In this report, we describe experiments to reveal design aspects of the selection strategy that favor site-selective and regioselective synthesis of native 3′-5′ RNA linkages. The results also reveal that an explicit selection pressure for RNA substrate sequence generality must be developed if the deoxyribozymes are to have practical generality.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 4391-4398 |
| Number of pages | 8 |
| Journal | Organic and Biomolecular Chemistry |
| Volume | 6 |
| Issue number | 23 |
| DOIs | |
| State | Published - Nov 24 2008 |
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ASJC Scopus subject areas
- Biochemistry
- Physical and Theoretical Chemistry
- Organic Chemistry
Cite this
Controlling the direction of site-selectivity and regioselectivity in RNA ligation by Zn2+-dependent deoxyribozymes that use 2′,3′-cyclic phosphate RNA substrates. / Kost, Diana M.; Gerdt, Joseph P.; Pradeepkumar, P. I.; Silverman, Scott K.
In: Organic and Biomolecular Chemistry, Vol. 6, No. 23, 24.11.2008, p. 4391-4398.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Controlling the direction of site-selectivity and regioselectivity in RNA ligation by Zn2+-dependent deoxyribozymes that use 2′,3′-cyclic phosphate RNA substrates
AU - Kost, Diana M.
AU - Gerdt, Joseph P.
AU - Pradeepkumar, P. I.
AU - Silverman, Scott K.
PY - 2008/11/24
Y1 - 2008/11/24
N2 - Our previous efforts have used in vitro selection to identify numerous Zn2+-dependent deoxyribozymes that ligate two RNA substrates with reaction at a 2′,3′-cyclic phosphate. Each deoxyribozyme creates one of several different RNA linkages, including native 3′-5′ and non-native 2′-5′ phosphodiester bonds as well as many unnatural linkages. In this report, we describe experiments to reveal design aspects of the selection strategy that favor site-selective and regioselective synthesis of native 3′-5′ RNA linkages. The results also reveal that an explicit selection pressure for RNA substrate sequence generality must be developed if the deoxyribozymes are to have practical generality.
AB - Our previous efforts have used in vitro selection to identify numerous Zn2+-dependent deoxyribozymes that ligate two RNA substrates with reaction at a 2′,3′-cyclic phosphate. Each deoxyribozyme creates one of several different RNA linkages, including native 3′-5′ and non-native 2′-5′ phosphodiester bonds as well as many unnatural linkages. In this report, we describe experiments to reveal design aspects of the selection strategy that favor site-selective and regioselective synthesis of native 3′-5′ RNA linkages. The results also reveal that an explicit selection pressure for RNA substrate sequence generality must be developed if the deoxyribozymes are to have practical generality.
UR - http://www.scopus.com/inward/record.url?scp=56349102235&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=56349102235&partnerID=8YFLogxK
U2 - 10.1039/b813566e
DO - 10.1039/b813566e
M3 - Article
C2 - 19005599
AN - SCOPUS:56349102235
VL - 6
SP - 4391
EP - 4398
JO - Organic and Biomolecular Chemistry
JF - Organic and Biomolecular Chemistry
SN - 1477-0520
IS - 23
ER -