Controlled vesicle deformation and lysis by single oscillating bubbles

Philippe Marmottant, Sascha Hilgenfeldt

Research output: Contribution to journalArticle

Abstract

The ability of collapsing (cavitating) bubbles to focus and concentrate energy, forces and stresses is at the root of phenomena such as cavitation damage, sonochemistry or sonoluminescence. In a biomedical context, ultrasound-driven microbubbles have been used to enhance contrast in ultrasonic images. The observation of bubble-enhanced sonoporation acoustically induced rupture of membranes - has also opened up intriguing possibilities for the therapeutic application of sonoporation as an alternative to cell-wall permeation techniques such as electroporation and particle guns. However, these pioneering experiments have not been able to pinpoint the mechanism by which the violently collapsing bubble opens pores or larger holes in membranes. Here we present an experiment in which gentle (linear) bubble oscillations are sufficient to achieve rupture of lipid membranes. In this regime, the bubble dynamics and the ensuing sonoporation can be accurately controlled. The use of microbubbles as focusing agents makes acoustics on the micrometre scale (microacoustics) a viable tool, with possible applications in cell manipulation and cellwall permeation as well as in microfluidic devices.

Original languageEnglish (US)
Pages (from-to)153-156
Number of pages4
JournalNature
Volume423
Issue number6936
DOIs
StatePublished - May 8 2003
Externally publishedYes

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Microbubbles
Lab-On-A-Chip Devices
Rupture
Electroporation
Membranes
Firearms
Membrane Lipids
Acoustics
Ultrasonics
Cell Wall
Observation
Therapeutics

ASJC Scopus subject areas

  • General

Cite this

Controlled vesicle deformation and lysis by single oscillating bubbles. / Marmottant, Philippe; Hilgenfeldt, Sascha.

In: Nature, Vol. 423, No. 6936, 08.05.2003, p. 153-156.

Research output: Contribution to journalArticle

Marmottant, Philippe ; Hilgenfeldt, Sascha. / Controlled vesicle deformation and lysis by single oscillating bubbles. In: Nature. 2003 ; Vol. 423, No. 6936. pp. 153-156.
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