Control of Protein Orientation on Gold Nanoparticles

Wayne Lin; Thomas Insley; Marcus D. Tuttle; Lingyang Zhu; Deborah A. Berthold; Petr Král; Chad M. Rienstra; Catherine J. Murphy

doi:10.1021/acs.jpcc.5b07701

Control of Protein Orientation on Gold Nanoparticles

Wayne Lin, Thomas Insley, Marcus D. Tuttle, Lingyang Zhu, Deborah A. Berthold, Petr Král, Chad M. Rienstra, Catherine J. Murphy

Research output: Contribution to journal › Article › peer-review

Abstract

Gold nanoparticles (Au NPs) have attracted much attention due to their potential applications in nanomedicine. While numerous studies have quantified biomolecular adsorption to Au NPs in terms of equilibrium binding constants, far less is known about biomolecular orientation on nanoparticle surfaces. In this study, the binding of the protein α-synuclein to citrate and (16-mercaptohexadecyl)trimethylammonium bromide (MTAB)-coated 12 nm Au NPs is examined by heteronuclear single quantum coherence NMR spectroscopy to provide site-specific measurements of protein-nanoparticle binding. Molecular dynamics simulations support the orientation assignments, which show N-terminus binding to the Au NP for citrate-capped NPs and C-terminus binding for the MTAB-capped NPs.

Original language	English (US)
Pages (from-to)	21035-21043
Number of pages	9
Journal	Journal of Physical Chemistry C
Volume	119
Issue number	36
DOIs	https://doi.org/10.1021/acs.jpcc.5b07701
State	Published - Sep 10 2015

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Energy(all)
Physical and Theoretical Chemistry
Surfaces, Coatings and Films

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10.1021/acs.jpcc.5b07701

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abstract = "Gold nanoparticles (Au NPs) have attracted much attention due to their potential applications in nanomedicine. While numerous studies have quantified biomolecular adsorption to Au NPs in terms of equilibrium binding constants, far less is known about biomolecular orientation on nanoparticle surfaces. In this study, the binding of the protein α-synuclein to citrate and (16-mercaptohexadecyl)trimethylammonium bromide (MTAB)-coated 12 nm Au NPs is examined by heteronuclear single quantum coherence NMR spectroscopy to provide site-specific measurements of protein-nanoparticle binding. Molecular dynamics simulations support the orientation assignments, which show N-terminus binding to the Au NP for citrate-capped NPs and C-terminus binding for the MTAB-capped NPs.",

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AU - Lin, Wayne

AU - Insley, Thomas

AU - Tuttle, Marcus D.

AU - Zhu, Lingyang

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AU - Král, Petr

AU - Rienstra, Chad M.

AU - Murphy, Catherine J.

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Control of Protein Orientation on Gold Nanoparticles

Abstract

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