Control of Protein Orientation on Gold Nanoparticles

Wayne Lin, Thomas Insley, Marcus D. Tuttle, Lingyang Zhu, Deborah A. Berthold, Petr Král, Chad M. Rienstra, Catherine J. Murphy

Research output: Contribution to journalArticlepeer-review


Gold nanoparticles (Au NPs) have attracted much attention due to their potential applications in nanomedicine. While numerous studies have quantified biomolecular adsorption to Au NPs in terms of equilibrium binding constants, far less is known about biomolecular orientation on nanoparticle surfaces. In this study, the binding of the protein α-synuclein to citrate and (16-mercaptohexadecyl)trimethylammonium bromide (MTAB)-coated 12 nm Au NPs is examined by heteronuclear single quantum coherence NMR spectroscopy to provide site-specific measurements of protein-nanoparticle binding. Molecular dynamics simulations support the orientation assignments, which show N-terminus binding to the Au NP for citrate-capped NPs and C-terminus binding for the MTAB-capped NPs.

Original languageEnglish (US)
Pages (from-to)21035-21043
Number of pages9
JournalJournal of Physical Chemistry C
Issue number36
StatePublished - Sep 10 2015

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • General Energy
  • Physical and Theoretical Chemistry
  • Surfaces, Coatings and Films


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