Control of Nucleophile Chemoselectivity in Cyanobactin YcaO Heterocyclases PatD and TruD

Wenjia Gu, Yiwu Zheng, Taras Pogorelov, Satish K. Nair, Eric W. Schmidt

Research output: Contribution to journalArticlepeer-review

Abstract

Members of the YcaO superfamily are among the most common post-translational modification enzymes in natural product biosynthesis, with wide usage in biotechnology and synthetic biology applications. Here, we use domain-swapped chimeras and discovered unstructured regions in cyanobactin YcaOs that guide interactions with the substrates, governing access to interior amino acids in the substrates and explaining the chemoselectivity between PatD and TruD. These results define how the cyanobactin heterocyclases modify exceptionally sequence diverse substrates, yet with a high degree of positional and nucleophile selectivity.

Original languageEnglish (US)
Pages (from-to)1215–1225
JournalACS chemical biology
Volume17
Issue number5
Early online dateApr 14 2022
DOIs
StatePublished - May 20 2022

ASJC Scopus subject areas

  • Molecular Medicine
  • Biochemistry

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