Abstract

Capillary electrophoresis (CE) is a small-volume separation approach amenable to the analysis of complex samples for their small molecule, peptide and protein content. A number of the features of CE make it a method of choice for addressing questions related to neurochemistry. The figures of merit inherent to CE that make it well suited for studying cell-to-cell and intracellular signaling include small sample volumes, high separation efficiency, the ability for online analyte concentration, and compatibility with sensitive and high-information content detection methods. A variety of instrumental aspects are detailed, including detection methods and sampling techniques that are particularly useful for the analysis of signaling molecules. Studies that have used these techniques to increase our understanding of neurobiology are emphasized throughout. One notable application is single neuron chemical analysis, a research area that has been greatly advanced by CE.

Original languageEnglish (US)
Pages (from-to)144-158
Number of pages15
JournalJournal of Chromatography A
Volume1184
Issue number1-2
DOIs
StatePublished - Mar 14 2008

Fingerprint

Capillary electrophoresis
Molecules
Neurons
Sampling
Peptides
Chemical analysis
Proteins

Keywords

  • Capillary electrophoresis
  • Cell-to-cell signaling
  • Microdialysis
  • Neuronal release
  • Neuroscience
  • Neurotransmitters
  • Single cell analysis

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Contributions of capillary electrophoresis to neuroscience. / Lapainis, Theodore; Sweedler, Jonathan V.

In: Journal of Chromatography A, Vol. 1184, No. 1-2, 14.03.2008, p. 144-158.

Research output: Contribution to journalReview article

@article{d2cab21a12c042828578d65bd5ad5fb7,
title = "Contributions of capillary electrophoresis to neuroscience",
abstract = "Capillary electrophoresis (CE) is a small-volume separation approach amenable to the analysis of complex samples for their small molecule, peptide and protein content. A number of the features of CE make it a method of choice for addressing questions related to neurochemistry. The figures of merit inherent to CE that make it well suited for studying cell-to-cell and intracellular signaling include small sample volumes, high separation efficiency, the ability for online analyte concentration, and compatibility with sensitive and high-information content detection methods. A variety of instrumental aspects are detailed, including detection methods and sampling techniques that are particularly useful for the analysis of signaling molecules. Studies that have used these techniques to increase our understanding of neurobiology are emphasized throughout. One notable application is single neuron chemical analysis, a research area that has been greatly advanced by CE.",
keywords = "Capillary electrophoresis, Cell-to-cell signaling, Microdialysis, Neuronal release, Neuroscience, Neurotransmitters, Single cell analysis",
author = "Theodore Lapainis and Sweedler, {Jonathan V}",
year = "2008",
month = "3",
day = "14",
doi = "10.1016/j.chroma.2007.10.098",
language = "English (US)",
volume = "1184",
pages = "144--158",
journal = "Journal of Chromatography A",
issn = "0021-9673",
number = "1-2",

}

TY - JOUR

T1 - Contributions of capillary electrophoresis to neuroscience

AU - Lapainis, Theodore

AU - Sweedler, Jonathan V

PY - 2008/3/14

Y1 - 2008/3/14

N2 - Capillary electrophoresis (CE) is a small-volume separation approach amenable to the analysis of complex samples for their small molecule, peptide and protein content. A number of the features of CE make it a method of choice for addressing questions related to neurochemistry. The figures of merit inherent to CE that make it well suited for studying cell-to-cell and intracellular signaling include small sample volumes, high separation efficiency, the ability for online analyte concentration, and compatibility with sensitive and high-information content detection methods. A variety of instrumental aspects are detailed, including detection methods and sampling techniques that are particularly useful for the analysis of signaling molecules. Studies that have used these techniques to increase our understanding of neurobiology are emphasized throughout. One notable application is single neuron chemical analysis, a research area that has been greatly advanced by CE.

AB - Capillary electrophoresis (CE) is a small-volume separation approach amenable to the analysis of complex samples for their small molecule, peptide and protein content. A number of the features of CE make it a method of choice for addressing questions related to neurochemistry. The figures of merit inherent to CE that make it well suited for studying cell-to-cell and intracellular signaling include small sample volumes, high separation efficiency, the ability for online analyte concentration, and compatibility with sensitive and high-information content detection methods. A variety of instrumental aspects are detailed, including detection methods and sampling techniques that are particularly useful for the analysis of signaling molecules. Studies that have used these techniques to increase our understanding of neurobiology are emphasized throughout. One notable application is single neuron chemical analysis, a research area that has been greatly advanced by CE.

KW - Capillary electrophoresis

KW - Cell-to-cell signaling

KW - Microdialysis

KW - Neuronal release

KW - Neuroscience

KW - Neurotransmitters

KW - Single cell analysis

UR - http://www.scopus.com/inward/record.url?scp=39749178511&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=39749178511&partnerID=8YFLogxK

U2 - 10.1016/j.chroma.2007.10.098

DO - 10.1016/j.chroma.2007.10.098

M3 - Review article

C2 - 18054026

AN - SCOPUS:39749178511

VL - 1184

SP - 144

EP - 158

JO - Journal of Chromatography A

JF - Journal of Chromatography A

SN - 0021-9673

IS - 1-2

ER -