A method is derived to evaluate the fluorescence intensity which is observed in photobleaching experiments involving a time-independent sin2kx grating. This grating corresponds to a local (position x) rate constant rsin2kx of photobleaching. Application of the method, demonstrates how such experiments can yield information on lateral diffusion in artificial and biological membranes. A simple analytical approximation for the fluorescence intensity which holds for a time period less than 10/r is provided.
ASJC Scopus subject areas
- Physics and Astronomy(all)
- Physical and Theoretical Chemistry