Consumption of N2O and other n-cycle intermediates by gemmatimonas aurantiaca strain T-27

Joanne Chee-Sanford, David Tian, Robert Sanford

Research output: Contribution to journalArticlepeer-review

Abstract

Bacteria affiliated with the phylum Gemmatimonadetes are found in high abundance in many terrestrial and aquatic environments, yet little is known about their metabolic capabilities. Difficulty in their cultivation has prompted interest in identifying better growth conditions for metabolic studies, especially related to their ability to reduce N2 O, a potent greenhouse gas. T-27 Gemmatimonas aurantiaca is one of few cultivated strains of Gemmatimonadetes available for physiological studies. Our objective was to test this organism’s ability to use nitrite, nitrate, and N2 O, and mineral forms of assimilable NH4 + at concentrations not typically used in tests for compound utilization. Cultures incubated under anaerobic conditions with nitrate, nitrite or N2 O failed to grow or show depletion of these substrates. Nitrate and nitrite (1 mM) were not used even when cells were grown aerobically with the O2 allowed to deplete first. N2 O reduction only commenced in the presence of O2 and continued to be depleted when refed to the culture under anaerobic, microaerobic and aerobic atmospheres. Carbon mineralization was coupled to the electron-accepting processes, with higher reducing equivalents needed for N2 O utilization under aerobic atmospheres. N2 O was reduced to N2 in the presence of 20% O2, however the rate of this reaction is reduced in the presence of high O2 concentration. This study demonstrated that G. aurantiaca T-27 possesses unique characteristics for assimilative and dissimilative N processes with new implications for cultivation strategies to better assess the metabolic abilities of Gemmatimonadetes.

Original languageEnglish (US)
Article number000847
Pages (from-to)1345-1354
Number of pages10
JournalMicrobiology (United Kingdom)
Volume165
Issue number12
DOIs
StatePublished - 2019

Keywords

  • Clade II nosZ
  • Gemmatimonas aurantiaca
  • N-oxide reduction

ASJC Scopus subject areas

  • Microbiology

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