Construction and characterization of an ovine BAC contig spanning the callipyge locus

K. Segers, S. Berghmans, M. Georges, C. Charlier, D. Vaiman, T. Shay, N. Cockett, S. Meyers, J. Beever

Research output: Contribution to journalArticlepeer-review


We describe the construction of an ovine BAC contig spanning a 4·6 centimorgan (cM) chromosome segment known to contain the callipyge (CLPG) locus. The contig comprises 21 ovine BAC clones jointly covering approximately 900 kilobases (Kb). Two gaps in the BAC contig, spanning 10 and 7·5 Kb, respectively, were bridged by long range PCR. The corresponding chromosome region was shown to be characterized by an unusually low Kb to cM ratio (164 Kb/cM) and a high density of NotI sites (1:126 Kb) possibly reflecting a high gene density in the corresponding chromosome region. Equivalent amplification of 64 sequence tagged sites spanning the corresponding region from homozygous + / + and CLPG/CLPG individuals disproves the hypothesis of a major deletion causing the CLPG mutation.

Original languageEnglish (US)
Pages (from-to)352-359
Number of pages8
JournalAnimal genetics
Issue number6
StatePublished - 2000
Externally publishedYes


  • Callipyge locus
  • Long range PCR
  • Ovine BAC contig

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Genetics


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