Construction and characterization of a recombinant adenovirus directing expression of the MAGE-1 tumor-specific antigen

Darryl S. Reed, Pedro Romero, Donata Rimoldi, Jean Charles Cerottini, Jerome Schaack, C. Victor Jongeneel

Research output: Contribution to journalArticlepeer-review


The finding that many human melanomas express distinct antigens that can be recognised by specific cytolytic T lymphocytes (CTL) implies that immunotherapeutic strategies against this cancer might prove effective. The ex vivo delivery of a tumour-associated antigen to autologous cells and the subsequent re-administration of these cells to the patient might prove effective in boosting the T cell immune response. Recombinant human adenoviral vectors provide an efficient delivery system and have many advantages over other viral and non-viral delivery vehicles. Infection of a panel of human melanoma cell lines by AdCMVMAGE-1, a novel recombinant adenovirus which incorporates the full-length MAGE-1 cDNA, was shown to induce production of high levels of MAGE-1 protein. Incubation of transduced HLA-AI expressing melanoma cell lines with 2 anti-MAGE-1.A1 CTL clones resulted in specific recognition and lysis of target cells, indicating that the exogenous MAGE-1 protein was processed and presented in a normal manner. Furthermore, quantitative analyses demonstrated a correlation between the efficiency of transduction and the proportion of cells lysed. Importantly for future clinical trials, stimulation of peripheral blood lymphocytes (PBLs) from a melanoma patient by AdCMVMAGE- 1-transduced autologous cells resulted in the generation of specific CTLs against the MAGE-1 antigen. Together, our data emphasize the utility of adenoviruses as vaccination vehicles and highlight the potential efficacy of this approach for the treatment of melanoma.

Original languageEnglish (US)
Pages (from-to)1045-1055
Number of pages11
JournalInternational Journal of Cancer
Issue number6
StatePublished - Sep 17 1997
Externally publishedYes

ASJC Scopus subject areas

  • Oncology
  • Cancer Research


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