Construction and characterization of a full-length cDNA infectious clone of emerging porcine Senecavirus A

Zhenhai Chen; Fangfeng Yuan; Yanhua Li; Pengcheng Shang; Robin Schroeder; Kelly Lechtenberg; Jamie Henningson; Benjamin Hause; Jianfa Bai; Raymond R.R. Rowland; Alfonso Clavijo; Ying Fang

doi:10.1016/j.virol.2016.07.003

Construction and characterization of a full-length cDNA infectious clone of emerging porcine Senecavirus A

Zhenhai Chen, Fangfeng Yuan, Yanhua Li, Pengcheng Shang, Robin Schroeder, Kelly Lechtenberg, Jamie Henningson, Benjamin Hause, Jianfa Bai, Raymond R.R. Rowland, Alfonso Clavijo, Ying Fang

Research output: Contribution to journal › Article › peer-review

Abstract

A full-length cDNA infectious clone, pKS15-01-Clone, was constructed from an emerging Senecavirus A (SVA; strain KS15-01). To explore the potential use as a viral backbone for expressing marker genes, the enhanced green fluorescent protein (EGFP)-tagged reporter virus (vKS15-01-EGFP) was generated using reverse genetics. Compared to the parental virus, the pKS15-01-Clone derived virus (vKS15-01-Clone) replicated efficiently in vitro and in vivo, and induced similar levels of neutralizing antibody and cytokine responses in infected animals. In contrast, the vKS15-01-EGFP virus showed impaired growth ability and induced lower level of immune response in infected animals. Lesions on the dorsal snout and coronary bands were observed in all pigs infected by parental virus KS15-01, but not in pigs infected with vKS15-01-Clone or vKS15-01-EGFP viruses. These results demonstrated that the infectious clone and EGFP reporter virus could be used as important tools in further elucidating the SVA pathogenesis and development of control measures.

Original language	English (US)
Pages (from-to)	111-124
Number of pages	14
Journal	Virology
Volume	497
DOIs	https://doi.org/10.1016/j.virol.2016.07.003
State	Published - Oct 1 2016
Externally published	Yes

Keywords

EGFP reporter virus
Infectious clone
Picornavirus infection
Reverse genetics
SVA pathogenesis
Senecavirus A
Swine
Vesicular disease
Vesicular lesion

ASJC Scopus subject areas

Virology

Online availability

10.1016/j.virol.2016.07.003

Library availability

Discover UIUC Full Text

Cite this

@article{13e41423ac714dbe8eb728a9ee70ff51,

title = "Construction and characterization of a full-length cDNA infectious clone of emerging porcine Senecavirus A",

abstract = "A full-length cDNA infectious clone, pKS15-01-Clone, was constructed from an emerging Senecavirus A (SVA; strain KS15-01). To explore the potential use as a viral backbone for expressing marker genes, the enhanced green fluorescent protein (EGFP)-tagged reporter virus (vKS15-01-EGFP) was generated using reverse genetics. Compared to the parental virus, the pKS15-01-Clone derived virus (vKS15-01-Clone) replicated efficiently in vitro and in vivo, and induced similar levels of neutralizing antibody and cytokine responses in infected animals. In contrast, the vKS15-01-EGFP virus showed impaired growth ability and induced lower level of immune response in infected animals. Lesions on the dorsal snout and coronary bands were observed in all pigs infected by parental virus KS15-01, but not in pigs infected with vKS15-01-Clone or vKS15-01-EGFP viruses. These results demonstrated that the infectious clone and EGFP reporter virus could be used as important tools in further elucidating the SVA pathogenesis and development of control measures.",

keywords = "EGFP reporter virus, Infectious clone, Picornavirus infection, Reverse genetics, SVA pathogenesis, Senecavirus A, Swine, Vesicular disease, Vesicular lesion",

author = "Zhenhai Chen and Fangfeng Yuan and Yanhua Li and Pengcheng Shang and Robin Schroeder and Kelly Lechtenberg and Jamie Henningson and Benjamin Hause and Jianfa Bai and Rowland, {Raymond R.R.} and Alfonso Clavijo and Ying Fang",

note = "Publisher Copyright: {\textcopyright} 2016 Elsevier Inc.",

year = "2016",

month = oct,

day = "1",

doi = "10.1016/j.virol.2016.07.003",

language = "English (US)",

volume = "497",

pages = "111--124",

journal = "Virology",

issn = "0042-6822",

publisher = "Academic Press Inc.",

}

TY - JOUR

T1 - Construction and characterization of a full-length cDNA infectious clone of emerging porcine Senecavirus A

AU - Chen, Zhenhai

AU - Yuan, Fangfeng

AU - Li, Yanhua

AU - Shang, Pengcheng

AU - Schroeder, Robin

AU - Lechtenberg, Kelly

AU - Henningson, Jamie

AU - Hause, Benjamin

AU - Bai, Jianfa

AU - Rowland, Raymond R.R.

AU - Clavijo, Alfonso

AU - Fang, Ying

PY - 2016/10/1

Y1 - 2016/10/1

N2 - A full-length cDNA infectious clone, pKS15-01-Clone, was constructed from an emerging Senecavirus A (SVA; strain KS15-01). To explore the potential use as a viral backbone for expressing marker genes, the enhanced green fluorescent protein (EGFP)-tagged reporter virus (vKS15-01-EGFP) was generated using reverse genetics. Compared to the parental virus, the pKS15-01-Clone derived virus (vKS15-01-Clone) replicated efficiently in vitro and in vivo, and induced similar levels of neutralizing antibody and cytokine responses in infected animals. In contrast, the vKS15-01-EGFP virus showed impaired growth ability and induced lower level of immune response in infected animals. Lesions on the dorsal snout and coronary bands were observed in all pigs infected by parental virus KS15-01, but not in pigs infected with vKS15-01-Clone or vKS15-01-EGFP viruses. These results demonstrated that the infectious clone and EGFP reporter virus could be used as important tools in further elucidating the SVA pathogenesis and development of control measures.

AB - A full-length cDNA infectious clone, pKS15-01-Clone, was constructed from an emerging Senecavirus A (SVA; strain KS15-01). To explore the potential use as a viral backbone for expressing marker genes, the enhanced green fluorescent protein (EGFP)-tagged reporter virus (vKS15-01-EGFP) was generated using reverse genetics. Compared to the parental virus, the pKS15-01-Clone derived virus (vKS15-01-Clone) replicated efficiently in vitro and in vivo, and induced similar levels of neutralizing antibody and cytokine responses in infected animals. In contrast, the vKS15-01-EGFP virus showed impaired growth ability and induced lower level of immune response in infected animals. Lesions on the dorsal snout and coronary bands were observed in all pigs infected by parental virus KS15-01, but not in pigs infected with vKS15-01-Clone or vKS15-01-EGFP viruses. These results demonstrated that the infectious clone and EGFP reporter virus could be used as important tools in further elucidating the SVA pathogenesis and development of control measures.

KW - EGFP reporter virus

KW - Infectious clone

KW - Picornavirus infection

KW - Reverse genetics

KW - SVA pathogenesis

KW - Senecavirus A

KW - Swine

KW - Vesicular disease

KW - Vesicular lesion

UR - http://www.scopus.com/inward/record.url?scp=84979076034&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84979076034&partnerID=8YFLogxK

U2 - 10.1016/j.virol.2016.07.003

DO - 10.1016/j.virol.2016.07.003

M3 - Article

C2 - 27459668

AN - SCOPUS:84979076034

SN - 0042-6822

VL - 497

SP - 111

EP - 124

JO - Virology

JF - Virology

ER -

Construction and characterization of a full-length cDNA infectious clone of emerging porcine Senecavirus A

Abstract

Keywords

ASJC Scopus subject areas

Online availability

Library availability

Related links

Fingerprint

Cite this