Consolidated sustainable organic nanozyme integrated with Point-Of-Use sensing platform for dual agricultural and biological molecule detection

The newly introduced organic nanozymes offer a promising alternative to inorganic nanozymes with sustainable and cost-effective solutions. Given the potential of nanozyme-based Point-of-Care (PoC) systems for on-demand molecule sensing, these user-friendly Point-of-Use (PoU) platforms can be highly beneficial for real-world agricultural and biological applications. Herein, a novel, fully organic compound/monomer-based, sustainable organic nanozyme that exhibits peroxidase-like enzyme-like catalytic activity is introduced. Utilizing a unique self-assembled particle fabrication process, homogenous nanozyme are fabricated within a short time (within 50 nm, D₉₀), exhibiting decent kinetic profiles (K_m = 0.008 mM, H₂O₂), and degradability for improved waste management after its use. OM nanozyme-incorporated colorimetric sensing platforms successfully detect popular agricultural toxic biomolecules and biological molecules while achieving decent molecule sensing performance (Limit of Detection (LOD), glyphosate: 0.290 pgmL⁻¹, glucose: 3.47 ngmL⁻¹) with high analytical sensitivity and selectivity. A smartphone image-based polynomial regression analysis system and paper-microfluidic hardware were also developed to facilitate PoU applications in a lab-free environment with satisfactory analytic sensitivity (at least 1.6 μgmL⁻¹ for glyphosate and glucose). This sustainable OM nanozyme, coupled with the PoU platform, is envisioned to have broad applications in agricultural and biological systems.