Abstract

Multiplexing is a method of analyzing multiple analytes in a biological assay in a single step. Multiplexing provides advantages of sample sparring, shorter time to result and reduce tests cost. To achieve multiplexing we have used barcoded particles which were fabricated by a Stop Flow Lithography process in a microfluidic environment. Here, we present a microfluidic system for electrical differentiation of barcoded particles and its sensitivity to enumerate blood cells. The barcoded particles conjugated with different sized microspheres simulating blood cells generated distinct electrical signatures when passed through a microfluidic coulter counter, highlighting its ability for multiplexed analyte quantification. Such multiplexing system can be used for detecting multiple diagnostics and prognostic biomarkers in diseases like Sepsis, Acute Kidney Injury, and AIDS diagnostic and management.

Original languageEnglish (US)
Title of host publication2019 IEEE Healthcare Innovations and Point of Care Technologies, HI-POCT 2019
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages139-142
Number of pages4
ISBN (Electronic)9781728138121
DOIs
StatePublished - Nov 2019
Event2019 IEEE Healthcare Innovations and Point of Care Technologies, HI-POCT 2019 - Bethesda, United States
Duration: Nov 20 2019Nov 22 2019

Publication series

Name2019 IEEE Healthcare Innovations and Point of Care Technologies, HI-POCT 2019

Conference

Conference2019 IEEE Healthcare Innovations and Point of Care Technologies, HI-POCT 2019
CountryUnited States
CityBethesda
Period11/20/1911/22/19

ASJC Scopus subject areas

  • Artificial Intelligence
  • Computer Science Applications
  • Biomedical Engineering
  • Health Informatics
  • Instrumentation
  • Health(social science)

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  • Cite this

    Prakash, S., Nagarajan, M., Doyle, P., Bashir, R., & Hassan, U. (2019). Conjugated Barcoded Particles for Multiplexed Biomarker Quantification with a Microfluidic Biochip. In 2019 IEEE Healthcare Innovations and Point of Care Technologies, HI-POCT 2019 (pp. 139-142). [8962846] (2019 IEEE Healthcare Innovations and Point of Care Technologies, HI-POCT 2019). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/HI-POCT45284.2019.8962846