Conjugated Barcoded Particles for Multiplexed Biomarker Quantification with a Microfluidic Biochip

Shreya Prakash; Maxwell Nagarajan; Patrick Doyle; Rashid Bashir; Umer Hassan

doi:10.1109/HI-POCT45284.2019.8962846

Conjugated Barcoded Particles for Multiplexed Biomarker Quantification with a Microfluidic Biochip

Shreya Prakash, Maxwell Nagarajan, Patrick Doyle, Rashid Bashir, Umer Hassan

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

Multiplexing is a method of analyzing multiple analytes in a biological assay in a single step. Multiplexing provides advantages of sample sparring, shorter time to result and reduce tests cost. To achieve multiplexing we have used barcoded particles which were fabricated by a Stop Flow Lithography process in a microfluidic environment. Here, we present a microfluidic system for electrical differentiation of barcoded particles and its sensitivity to enumerate blood cells. The barcoded particles conjugated with different sized microspheres simulating blood cells generated distinct electrical signatures when passed through a microfluidic coulter counter, highlighting its ability for multiplexed analyte quantification. Such multiplexing system can be used for detecting multiple diagnostics and prognostic biomarkers in diseases like Sepsis, Acute Kidney Injury, and AIDS diagnostic and management.

Original language	English (US)
Title of host publication	2019 IEEE Healthcare Innovations and Point of Care Technologies, HI-POCT 2019
Publisher	Institute of Electrical and Electronics Engineers Inc.
Pages	139-142
Number of pages	4
ISBN (Electronic)	9781728138121
DOIs	https://doi.org/10.1109/HI-POCT45284.2019.8962846
State	Published - Nov 2019
Event	2019 IEEE Healthcare Innovations and Point of Care Technologies, HI-POCT 2019 - Bethesda, United States Duration: Nov 20 2019 → Nov 22 2019

Publication series

Name	2019 IEEE Healthcare Innovations and Point of Care Technologies, HI-POCT 2019

Conference

Conference	2019 IEEE Healthcare Innovations and Point of Care Technologies, HI-POCT 2019
Country/Territory	United States
City	Bethesda
Period	11/20/19 → 11/22/19

ASJC Scopus subject areas

Artificial Intelligence
Computer Science Applications
Biomedical Engineering
Health Informatics
Instrumentation
Health(social science)

Online availability

10.1109/HI-POCT45284.2019.8962846

Library availability

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Cite this

Prakash, S., Nagarajan, M., Doyle, P., Bashir, R., & Hassan, U. (2019). Conjugated Barcoded Particles for Multiplexed Biomarker Quantification with a Microfluidic Biochip. In 2019 IEEE Healthcare Innovations and Point of Care Technologies, HI-POCT 2019 (pp. 139-142). Article 8962846 (2019 IEEE Healthcare Innovations and Point of Care Technologies, HI-POCT 2019). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/HI-POCT45284.2019.8962846

Conjugated Barcoded Particles for Multiplexed Biomarker Quantification with a Microfluidic Biochip. / Prakash, Shreya; Nagarajan, Maxwell; Doyle, Patrick et al.
2019 IEEE Healthcare Innovations and Point of Care Technologies, HI-POCT 2019. Institute of Electrical and Electronics Engineers Inc., 2019. p. 139-142 8962846 (2019 IEEE Healthcare Innovations and Point of Care Technologies, HI-POCT 2019).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Prakash, S, Nagarajan, M, Doyle, P, Bashir, R & Hassan, U 2019, Conjugated Barcoded Particles for Multiplexed Biomarker Quantification with a Microfluidic Biochip. in 2019 IEEE Healthcare Innovations and Point of Care Technologies, HI-POCT 2019., 8962846, 2019 IEEE Healthcare Innovations and Point of Care Technologies, HI-POCT 2019, Institute of Electrical and Electronics Engineers Inc., pp. 139-142, 2019 IEEE Healthcare Innovations and Point of Care Technologies, HI-POCT 2019, Bethesda, United States, 11/20/19. https://doi.org/10.1109/HI-POCT45284.2019.8962846

Prakash S, Nagarajan M, Doyle P, Bashir R, Hassan U. Conjugated Barcoded Particles for Multiplexed Biomarker Quantification with a Microfluidic Biochip. In 2019 IEEE Healthcare Innovations and Point of Care Technologies, HI-POCT 2019. Institute of Electrical and Electronics Engineers Inc. 2019. p. 139-142. 8962846. (2019 IEEE Healthcare Innovations and Point of Care Technologies, HI-POCT 2019). doi: 10.1109/HI-POCT45284.2019.8962846

Prakash, Shreya ; Nagarajan, Maxwell ; Doyle, Patrick et al. / Conjugated Barcoded Particles for Multiplexed Biomarker Quantification with a Microfluidic Biochip. 2019 IEEE Healthcare Innovations and Point of Care Technologies, HI-POCT 2019. Institute of Electrical and Electronics Engineers Inc., 2019. pp. 139-142 (2019 IEEE Healthcare Innovations and Point of Care Technologies, HI-POCT 2019).

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Conjugated Barcoded Particles for Multiplexed Biomarker Quantification with a Microfluidic Biochip

Abstract

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