Multiplexing is a method of analyzing multiple analytes in a biological assay in a single step. Multiplexing provides advantages of sample sparring, shorter time to result and reduce tests cost. To achieve multiplexing we have used barcoded particles which were fabricated by a Stop Flow Lithography process in a microfluidic environment. Here, we present a microfluidic system for electrical differentiation of barcoded particles and its sensitivity to enumerate blood cells. The barcoded particles conjugated with different sized microspheres simulating blood cells generated distinct electrical signatures when passed through a microfluidic coulter counter, highlighting its ability for multiplexed analyte quantification. Such multiplexing system can be used for detecting multiple diagnostics and prognostic biomarkers in diseases like Sepsis, Acute Kidney Injury, and AIDS diagnostic and management.