Concord grape pomace polyphenols complexed to soy protein isolate are stable and hypoglycemic in diabetic mice

Diana E. Roopchand, Peter Kuhn, Christian G. Krueger, Kristin Moskal, Mary Ann Lila, Ilya Raskin

Research output: Contribution to journalArticlepeer-review

Abstract

Polyphenols extracted from Concord grape pomace were stabilized by complexation to soy protein isolate (SPI) to produce grape polyphenol-SPI complex (GP-SPI) containing 5% or 10% grape polyphenols. LC-MS and MALDI-TOF analysis showed that a broad range of phytochemicals were present in the grape pomace extract. Anthocyanins and total polyphenols in the GP-SPI complex were stable after a 16-week incubation at 37 C but were reduced by up 60% in dried grape pomace extract. Compared to vehicle (236 ± 34 mg/dL), a single dose of 300 mg/kg GP-SPI (184 ± 32 mg/dL) or 500 mg/kg GP-SPI (177 ± 28 mg/dL) having 5% grape polyphenols significantly lowered blood glucose in obese and hyperglycemic C57BL/6 mice 6 h after administration. GP-SPI allows the capture of grape pomace polyphenols in a protein-rich food matrix and may be useful as a functional food ingredient for the management of blood glucose levels.

Original languageEnglish (US)
Pages (from-to)11428-11433
Number of pages6
JournalJournal of Agricultural and Food Chemistry
Volume61
Issue number47
DOIs
StatePublished - Nov 27 2013
Externally publishedYes

Keywords

  • grape pomace
  • hyperglycemia
  • nutrition
  • polyphenols
  • soy protein isolate

ASJC Scopus subject areas

  • Chemistry(all)
  • Agricultural and Biological Sciences(all)

Fingerprint Dive into the research topics of 'Concord grape pomace polyphenols complexed to soy protein isolate are stable and hypoglycemic in diabetic mice'. Together they form a unique fingerprint.

Cite this