Composition of ruminal bacteria harvested from steers as influenced by dietary forage level and fat supplementation.

H. S. Hussein, N. R. Merchen, G. C. Fahey

Research output: Contribution to journalArticlepeer-review


The objective of this study was to examine the effects of dietary forage level and fat supplementation on the chemical composition of mixed ruminal bacteria (MRB). Six ruminally cannulated beef steers (354 kg +/- 18) were given ad libitum access to six diets (13.2% CP; DM basis) that were offered twice daily in a 6 x 6 Latin square design. Treatments were arranged as a 2 x 3 factorial with two forage levels (70 vs 30% of dietary DM as corn silage) and three forms of fat supplementation including no canola seed or canola seed added at 10% of dietary DM as whole treated with alkaline hydrogen peroxide or untreated crushed. Canola seed contributed 5% added fat to the total diet. The remaining dietary ingredients were corn, canola meal, molasses, and urea. No interactions (P > .05) between dietary forage level and canola seed supplementation were observed. Concentrations of OM, N, and all amino acids were higher (P < .05) in MRB from steers fed low forage than in MRB from steers fed high forage. Concentrations of purines and GE and the N:purines ratio in MRB were not affected (P > .05) by dietary forage level or canola seed supplementation. Canola seed supplementation did not affect (P > .05) concentrations of OM, N, or most of the amino acids in MRB. Concentrations of four essential amino acids (i.e., isoleucine, leucine, lysine, and phenylalanine) in MRB were decreased (P < .05) due to canola seed supplementation. Dietary forage level did not affect (P > .05) concentrations of long-chain fatty acids in MRB.(ABSTRACT TRUNCATED AT 250 WORDS)

Original languageEnglish (US)
Pages (from-to)2469-2473
Number of pages5
JournalJournal of animal science
Issue number8
StatePublished - Aug 1995

ASJC Scopus subject areas

  • Food Science
  • Animal Science and Zoology
  • Genetics


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