Complex structures of MoeN5 with substrate analogues suggest sequential catalytic mechanism

Lilan Zhang, Tzu Ping Ko, Satish R. Malwal, Weidong Liu, Shuyu Zhou, Xuejing Yu, Eric Oldfield, Rey Ting Guo, Chun Chi Chen

Research output: Contribution to journalArticle

Abstract

The antibiotic moenomycin A is a phosphoglycerate derivative with a C 25 -moenocinyl chain and a branched oligosaccharide. Formation of the C 25 -chain is catalyzed by the enzyme MoeN5 with geranyl pyrophosphate (GPP) and the sugar-linked 2-Z,E-farnesyl-3-phosphoglycerate (FPG) as its substrates. Previous complex crystal structures with GPP and long-chain alkyl glycosides suggested that GPP binds to the S1 site in a similar way as in most other α-helical prenyltransferases (PTs), and FPG is likely to assume a bent conformation in the S2 site. However, two FPG derivatives synthesized in the current study were found in the S1 site rather than S2 in their complex crystal structures with MoeN5. Apparently S1 is the preferred site for prenyl-containing ligand, and S2 binding may proceed only after S1 is occupied. Thus, like most trans-type PTs, MoeN5 may employ a sequential ionization-condensation-elimination mechanism that involves a carbocation intermediate.

Original languageEnglish (US)
Pages (from-to)800-805
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume511
Issue number4
DOIs
StatePublished - Apr 16 2019

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Dimethylallyltranstransferase
Branched-Chain Oligosaccharides
Substrates
Crystal structure
Derivatives
Glycosides
Oligosaccharides
Sugars
Ionization
Conformations
Condensation
Anti-Bacterial Agents
Ligands
Enzymes
3-phosphoglycerate
geranyl pyrophosphate

Keywords

  • Antibiotics biosynthesis
  • Prenyltransferase
  • Sso7d protein tag
  • X-ray crystallography
  • moenomycin

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Complex structures of MoeN5 with substrate analogues suggest sequential catalytic mechanism. / Zhang, Lilan; Ko, Tzu Ping; Malwal, Satish R.; Liu, Weidong; Zhou, Shuyu; Yu, Xuejing; Oldfield, Eric; Guo, Rey Ting; Chen, Chun Chi.

In: Biochemical and Biophysical Research Communications, Vol. 511, No. 4, 16.04.2019, p. 800-805.

Research output: Contribution to journalArticle

Zhang, Lilan ; Ko, Tzu Ping ; Malwal, Satish R. ; Liu, Weidong ; Zhou, Shuyu ; Yu, Xuejing ; Oldfield, Eric ; Guo, Rey Ting ; Chen, Chun Chi. / Complex structures of MoeN5 with substrate analogues suggest sequential catalytic mechanism. In: Biochemical and Biophysical Research Communications. 2019 ; Vol. 511, No. 4. pp. 800-805.
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AU - Yu, Xuejing

AU - Oldfield, Eric

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