Complementary fluorescence-polarization microscopy using division-of-focal-plane polarization imaging sensor

Yang Liu, Timothy York, Walter Akers, Gail Sudlow, Viktor Gruev, Samuel Achilefu

Research output: Contribution to journalArticlepeer-review


Fluorescence microscopy offers high sensitivity for disease diagnosis. However, little structural information is revealed by this method, requiring another technique to localize the source of fluorescence. We developed a complementary fluorescence-polarization microscope. We used a division-of-focal-plane charge coupled device polarization sensor to enable real-time video rate polarization imaging without any moving parts. The polarization information provided by the microscope enabled detection of structural element and complements the fluorescence information. Application of this multimodal system for cancer imaging using a tumor selective molecular probe revealed the association of diminished structural integrity of tumor tissue with high fluorescence of the imaging agent compared to surrounding normal tissue. This study demonstrates a new paradigm to improve cancer detection and diagnosis.

Original languageEnglish (US)
Article number116001
JournalJournal of biomedical optics
Issue number11
StatePublished - Nov 2012
Externally publishedYes


  • fluorescence microscopy
  • molecular probe
  • near infrared
  • polarization microscopy
  • surgical margin
  • tumor imaging

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Biomedical Engineering


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