Compilation and analysis of intein sequences

Francine B. Perler, Gary J. Olsen, Eric Adam

Research output: Contribution to journalArticle

Abstract

We have compiled a list of all the inteins (protein splicing elements) whose sequences have been published or were available from on-line sequence databases as of September 18, 1996. Analysis of the 36 available intein sequences refines the previously described intein motifs and reveals the presence of another intein motif, Block H. Furthermore, analysis of the new inteins reshapes our view of the conserved splice junction residues, since three inteins lack the intein penultimate His seen in prior examples. Comparison of intein sequences suggests that, in general, (i) inteins present in the same location within extein homologs from different organisms are very closely related to each other in paired sequence comparison or phylogenetic analysis and we suggest that they should be considered intein alleles; (ii) multiple inteins present in the same gene are no more similar to each other than to inteins present in different genes; (iii) phylogenetic analysis indicates that inteins are so divergent that trees with statistically significant branches cannot be generated except for intein alleles.

Original languageEnglish (US)
Pages (from-to)1087-1093
Number of pages7
JournalNucleic acids research
Volume25
Issue number6
DOIs
StatePublished - Mar 15 1997

Fingerprint

Inteins
Sequence Analysis
Alleles
Protein Splicing
Matched-Pair Analysis

ASJC Scopus subject areas

  • Genetics

Cite this

Compilation and analysis of intein sequences. / Perler, Francine B.; Olsen, Gary J.; Adam, Eric.

In: Nucleic acids research, Vol. 25, No. 6, 15.03.1997, p. 1087-1093.

Research output: Contribution to journalArticle

Perler, Francine B. ; Olsen, Gary J. ; Adam, Eric. / Compilation and analysis of intein sequences. In: Nucleic acids research. 1997 ; Vol. 25, No. 6. pp. 1087-1093.
@article{f21178e72b0942c18ec7bb4cb7dc1611,
title = "Compilation and analysis of intein sequences",
abstract = "We have compiled a list of all the inteins (protein splicing elements) whose sequences have been published or were available from on-line sequence databases as of September 18, 1996. Analysis of the 36 available intein sequences refines the previously described intein motifs and reveals the presence of another intein motif, Block H. Furthermore, analysis of the new inteins reshapes our view of the conserved splice junction residues, since three inteins lack the intein penultimate His seen in prior examples. Comparison of intein sequences suggests that, in general, (i) inteins present in the same location within extein homologs from different organisms are very closely related to each other in paired sequence comparison or phylogenetic analysis and we suggest that they should be considered intein alleles; (ii) multiple inteins present in the same gene are no more similar to each other than to inteins present in different genes; (iii) phylogenetic analysis indicates that inteins are so divergent that trees with statistically significant branches cannot be generated except for intein alleles.",
author = "Perler, {Francine B.} and Olsen, {Gary J.} and Eric Adam",
year = "1997",
month = "3",
day = "15",
doi = "10.1093/nar/25.6.1087",
language = "English (US)",
volume = "25",
pages = "1087--1093",
journal = "Nucleic Acids Research",
issn = "0305-1048",
publisher = "Oxford University Press",
number = "6",

}

TY - JOUR

T1 - Compilation and analysis of intein sequences

AU - Perler, Francine B.

AU - Olsen, Gary J.

AU - Adam, Eric

PY - 1997/3/15

Y1 - 1997/3/15

N2 - We have compiled a list of all the inteins (protein splicing elements) whose sequences have been published or were available from on-line sequence databases as of September 18, 1996. Analysis of the 36 available intein sequences refines the previously described intein motifs and reveals the presence of another intein motif, Block H. Furthermore, analysis of the new inteins reshapes our view of the conserved splice junction residues, since three inteins lack the intein penultimate His seen in prior examples. Comparison of intein sequences suggests that, in general, (i) inteins present in the same location within extein homologs from different organisms are very closely related to each other in paired sequence comparison or phylogenetic analysis and we suggest that they should be considered intein alleles; (ii) multiple inteins present in the same gene are no more similar to each other than to inteins present in different genes; (iii) phylogenetic analysis indicates that inteins are so divergent that trees with statistically significant branches cannot be generated except for intein alleles.

AB - We have compiled a list of all the inteins (protein splicing elements) whose sequences have been published or were available from on-line sequence databases as of September 18, 1996. Analysis of the 36 available intein sequences refines the previously described intein motifs and reveals the presence of another intein motif, Block H. Furthermore, analysis of the new inteins reshapes our view of the conserved splice junction residues, since three inteins lack the intein penultimate His seen in prior examples. Comparison of intein sequences suggests that, in general, (i) inteins present in the same location within extein homologs from different organisms are very closely related to each other in paired sequence comparison or phylogenetic analysis and we suggest that they should be considered intein alleles; (ii) multiple inteins present in the same gene are no more similar to each other than to inteins present in different genes; (iii) phylogenetic analysis indicates that inteins are so divergent that trees with statistically significant branches cannot be generated except for intein alleles.

UR - http://www.scopus.com/inward/record.url?scp=0030763705&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030763705&partnerID=8YFLogxK

U2 - 10.1093/nar/25.6.1087

DO - 10.1093/nar/25.6.1087

M3 - Article

C2 - 9092614

AN - SCOPUS:0030763705

VL - 25

SP - 1087

EP - 1093

JO - Nucleic Acids Research

JF - Nucleic Acids Research

SN - 0305-1048

IS - 6

ER -