Compartmentalization of NaCl in frozen solutions of antifreeze glycoproteins

Y. Lin; J. A. Raymond; J. G. Duman; A. L. DeVries

doi:10.1016/0011-2240(76)90115-2

Compartmentalization of NaCl in frozen solutions of antifreeze glycoproteins

Y. Lin, J. A. Raymond, J. G. Duman, A. L. DeVries

Research output: Contribution to journal › Article › peer-review

Abstract

The freezing behavior of NaCl solutions containing antifreeze glycoproteins from an Antarctic fish has been investigated to determine whether the glycoproteins prevent concentration of NaCl during freezing. Frozen NaCl solutions containing glycoproteins exhibit greater resistance to releasing their brine during centrifugation than NaCl solutions containing other cryoprotectants. With the aid of calorimetry this is shown to be caused not by an incorporation of the NaCl into the ice but by compartmentalization of the brine pockets. The compartmentalization is attributed to an unusual spicular structure that is imposed on the ice by glycoproteins.

Original language	English (US)
Pages (from-to)	334-340
Number of pages	7
Journal	Cryobiology
Volume	13
Issue number	3
DOIs	https://doi.org/10.1016/0011-2240(76)90115-2
State	Published - Jun 1976
Externally published	Yes

ASJC Scopus subject areas

Biochemistry, Genetics and Molecular Biology(all)
Agricultural and Biological Sciences(all)

Online availability

10.1016/0011-2240(76)90115-2

Library availability

Discover UIUC Full Text

Cite this

@article{f9ebdae9a0404924837568840af8cb1e,

title = "Compartmentalization of NaCl in frozen solutions of antifreeze glycoproteins",

abstract = "The freezing behavior of NaCl solutions containing antifreeze glycoproteins from an Antarctic fish has been investigated to determine whether the glycoproteins prevent concentration of NaCl during freezing. Frozen NaCl solutions containing glycoproteins exhibit greater resistance to releasing their brine during centrifugation than NaCl solutions containing other cryoprotectants. With the aid of calorimetry this is shown to be caused not by an incorporation of the NaCl into the ice but by compartmentalization of the brine pockets. The compartmentalization is attributed to an unusual spicular structure that is imposed on the ice by glycoproteins.",

author = "Y. Lin and Raymond, {J. A.} and Duman, {J. G.} and DeVries, {A. L.}",

note = "Funding Information: Received April 30, 1975. 1 Supported by National Institutes of Health, Grant No. I-Rl-GM 21127-01, and National Science Foundation, Grant No. OPP74-07917.",

year = "1976",

month = jun,

doi = "10.1016/0011-2240(76)90115-2",

language = "English (US)",

volume = "13",

pages = "334--340",

journal = "Cryobiology",

issn = "0011-2240",

publisher = "Academic Press Inc.",

number = "3",

}

TY - JOUR

T1 - Compartmentalization of NaCl in frozen solutions of antifreeze glycoproteins

AU - Lin, Y.

AU - Raymond, J. A.

AU - Duman, J. G.

AU - DeVries, A. L.

N1 - Funding Information: Received April 30, 1975. 1 Supported by National Institutes of Health, Grant No. I-Rl-GM 21127-01, and National Science Foundation, Grant No. OPP74-07917.

PY - 1976/6

Y1 - 1976/6

N2 - The freezing behavior of NaCl solutions containing antifreeze glycoproteins from an Antarctic fish has been investigated to determine whether the glycoproteins prevent concentration of NaCl during freezing. Frozen NaCl solutions containing glycoproteins exhibit greater resistance to releasing their brine during centrifugation than NaCl solutions containing other cryoprotectants. With the aid of calorimetry this is shown to be caused not by an incorporation of the NaCl into the ice but by compartmentalization of the brine pockets. The compartmentalization is attributed to an unusual spicular structure that is imposed on the ice by glycoproteins.

AB - The freezing behavior of NaCl solutions containing antifreeze glycoproteins from an Antarctic fish has been investigated to determine whether the glycoproteins prevent concentration of NaCl during freezing. Frozen NaCl solutions containing glycoproteins exhibit greater resistance to releasing their brine during centrifugation than NaCl solutions containing other cryoprotectants. With the aid of calorimetry this is shown to be caused not by an incorporation of the NaCl into the ice but by compartmentalization of the brine pockets. The compartmentalization is attributed to an unusual spicular structure that is imposed on the ice by glycoproteins.

UR - http://www.scopus.com/inward/record.url?scp=0017195432&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0017195432&partnerID=8YFLogxK

U2 - 10.1016/0011-2240(76)90115-2

DO - 10.1016/0011-2240(76)90115-2

M3 - Article

C2 - 1277872

AN - SCOPUS:0017195432

SN - 0011-2240

VL - 13

SP - 334

EP - 340

JO - Cryobiology

JF - Cryobiology

IS - 3

ER -

Compartmentalization of NaCl in frozen solutions of antifreeze glycoproteins

Abstract

ASJC Scopus subject areas

Online availability

Library availability

Related links

Fingerprint

Cite this