Abstract

Juvenile hormone from the hemolymph of adult worker honey bees of known age and behavioral status was extracted and analyzed by two different radioimmunoassays in two independent laboratoies. The assays are different in hapten attachment, radiolabeled tracer, and the method by which bound and unbound hormone are separated. Despite these differences in the methods, hormone determinations were in excellent agreement at lower levels (0–50 ng/ml) but diverged as the hormone concentrations increased (> 50 ng/ml). The relative changes are in good agreement, with a correlation coefficient of 0.97. © 1993 Wiley‐Liss, Inc.

Original languageEnglish (US)
Pages (from-to)147-152
Number of pages6
JournalArchives of Insect Biochemistry and Physiology
Volume23
Issue number3
DOIs
StatePublished - 1993

Keywords

  • Apis mellifera
  • hemolymph
  • honey bee
  • juvenile hormone
  • radioimmunoassay

ASJC Scopus subject areas

  • Physiology
  • Biochemistry
  • Insect Science

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