Comparison of two different in vivo models and an in vitro model for caloric determination of four novel fiber ingredients

Sarah Cervantes-Pahm, Brenda K. Knapp, Beob G. Kim, Yanhong Liu, Carl M. Parsons, George C. Fahey, Hans H. Stein

Research output: Contribution to journalArticlepeer-review

Abstract

The objective of this study was to compare two in vivo methods using pigs and roosters and an in vitro method for determining the caloric value of four fiber sources [i.e., two resistant starches (RS 60 and RS 75), soluble corn fiber (SCF 70), and pullulan]. Metabolizable energy (ME) in pigs and true metabolizable energy (TMEn) in roosters were determined by using 72 barrows and 24 roosters, respectively. A two-step in vitro procedure was used to quantify monosaccharides released. Results of the two in vivo experiments corresponded well with RS 75 having the least caloric value (7.55 MJ/kg in pigs; 6.19 MJ/kg in roosters) and pullulan having the greatest caloric value (12.21 MJ/kg in pigs; 13.94 MJ/kg in roosters). The caloric values for all the fiber ingredients were less (P < 0.05) than in MD both in pigs and in roosters. Despite some limitations, results of the in vitro procedure corresponded well with the in vivo experiments where the concentration of glucose hydrolyzed from RS 60, RS 75, and SCF 70, but not pullulan, was less (P < 0.05) than the concentration of glucose hydrolyzed from MD. However, the greatest accuracy was obtained in the in vivo experiments.

Original languageEnglish (US)
Pages (from-to)12374-12379
Number of pages6
JournalJournal of Agricultural and Food Chemistry
Volume61
Issue number50
DOIs
StatePublished - Dec 18 2013

Keywords

  • maltodextrin
  • metabolizable energy
  • pullulan
  • resistant starch
  • soluble corn fiber

ASJC Scopus subject areas

  • Chemistry(all)
  • Agricultural and Biological Sciences(all)

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