Comparison of the effect of chemical composition of anthocyanin-rich plant extracts on colon cancer cell proliferation and their potential mechanism of action using in vitro, in silico, and biochemical assays

Candice Mazewski, Katie Liang, Elvira Gonzalez de Mejia

Research output: Research - peer-reviewArticle

Abstract

The objective was to compare the anti-proliferative effect of anthocyanin-rich plant extracts on human colon cancer cells and determine their mechanism of action. Eleven extracts were tested: red (RG) and purple grape, purple sweet potato, purple carrot, black and purple bean, black lentil (BL), black peanut, sorghum (SH), black rice, and blue wheat. HCT-116 and HT-29 inhibition correlated with total phenolics (r = 0.87 and 0.77, respectively), delphinidin-3-O-glucoside concentration with HT-29 inhibition (r = 0.69). The concentration inhibition fifty (IC50) for BL, SH, RG on HT-29 and HCT-116 cell proliferation ranged 0.9–2.0 mg/mL. Extracts decreased expression of anti-apoptotic proteins (survivin, cIAP-2, XIAP), induced apoptosis, and arrested cells in G1. Anthocyanins exhibited tyrosine kinase inhibitory potential in silico and biochemically; cyanidin-3-O-glucoside had one of the highest binding affinities with all kinases, especially ABL1 (−8.5 kcal/mol). Cyanidin-3-O-glucoside and delphinidin-3-O-glucoside inhibited EGFR (IC50 = 0.10 and 2.37 µM, respectively). Cyanidin-3-O-glucoside was the most potent anthocyanin on kinase inhibition.

LanguageEnglish (US)
Pages378-388
Number of pages11
JournalFood Chemistry
Volume242
DOIs
StatePublished - Mar 1 2018

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Anthocyanins
Plant Extracts
Computer Simulation
Colonic Neoplasms
Action Potentials
Cell Proliferation
In Vitro Techniques
cyanidin 3-O-glucoside
colorectal neoplasms
plant extracts
glucosides
anthocyanins
mechanism of action
cell proliferation
chemical composition
assays
neoplasm cells
Chemical analysis
Lens Plant
Sorghum

Keywords

  • Anthocyanins
  • Apoptosis
  • Black lentil
  • Colon cancer
  • Red grape
  • Sorghum
  • Tyrosine kinase

ASJC Scopus subject areas

  • Analytical Chemistry
  • Food Science
  • Medicine(all)

Cite this

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title = "Comparison of the effect of chemical composition of anthocyanin-rich plant extracts on colon cancer cell proliferation and their potential mechanism of action using in vitro, in silico, and biochemical assays",
abstract = "The objective was to compare the anti-proliferative effect of anthocyanin-rich plant extracts on human colon cancer cells and determine their mechanism of action. Eleven extracts were tested: red (RG) and purple grape, purple sweet potato, purple carrot, black and purple bean, black lentil (BL), black peanut, sorghum (SH), black rice, and blue wheat. HCT-116 and HT-29 inhibition correlated with total phenolics (r = 0.87 and 0.77, respectively), delphinidin-3-O-glucoside concentration with HT-29 inhibition (r = 0.69). The concentration inhibition fifty (IC50) for BL, SH, RG on HT-29 and HCT-116 cell proliferation ranged 0.9–2.0 mg/mL. Extracts decreased expression of anti-apoptotic proteins (survivin, cIAP-2, XIAP), induced apoptosis, and arrested cells in G1. Anthocyanins exhibited tyrosine kinase inhibitory potential in silico and biochemically; cyanidin-3-O-glucoside had one of the highest binding affinities with all kinases, especially ABL1 (−8.5 kcal/mol). Cyanidin-3-O-glucoside and delphinidin-3-O-glucoside inhibited EGFR (IC50 = 0.10 and 2.37 µM, respectively). Cyanidin-3-O-glucoside was the most potent anthocyanin on kinase inhibition.",
keywords = "Anthocyanins, Apoptosis, Black lentil, Colon cancer, Red grape, Sorghum, Tyrosine kinase",
author = "Candice Mazewski and Katie Liang and {Gonzalez de Mejia}, Elvira",
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T1 - Comparison of the effect of chemical composition of anthocyanin-rich plant extracts on colon cancer cell proliferation and their potential mechanism of action using in vitro, in silico, and biochemical assays

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AU - Liang,Katie

AU - Gonzalez de Mejia,Elvira

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N2 - The objective was to compare the anti-proliferative effect of anthocyanin-rich plant extracts on human colon cancer cells and determine their mechanism of action. Eleven extracts were tested: red (RG) and purple grape, purple sweet potato, purple carrot, black and purple bean, black lentil (BL), black peanut, sorghum (SH), black rice, and blue wheat. HCT-116 and HT-29 inhibition correlated with total phenolics (r = 0.87 and 0.77, respectively), delphinidin-3-O-glucoside concentration with HT-29 inhibition (r = 0.69). The concentration inhibition fifty (IC50) for BL, SH, RG on HT-29 and HCT-116 cell proliferation ranged 0.9–2.0 mg/mL. Extracts decreased expression of anti-apoptotic proteins (survivin, cIAP-2, XIAP), induced apoptosis, and arrested cells in G1. Anthocyanins exhibited tyrosine kinase inhibitory potential in silico and biochemically; cyanidin-3-O-glucoside had one of the highest binding affinities with all kinases, especially ABL1 (−8.5 kcal/mol). Cyanidin-3-O-glucoside and delphinidin-3-O-glucoside inhibited EGFR (IC50 = 0.10 and 2.37 µM, respectively). Cyanidin-3-O-glucoside was the most potent anthocyanin on kinase inhibition.

AB - The objective was to compare the anti-proliferative effect of anthocyanin-rich plant extracts on human colon cancer cells and determine their mechanism of action. Eleven extracts were tested: red (RG) and purple grape, purple sweet potato, purple carrot, black and purple bean, black lentil (BL), black peanut, sorghum (SH), black rice, and blue wheat. HCT-116 and HT-29 inhibition correlated with total phenolics (r = 0.87 and 0.77, respectively), delphinidin-3-O-glucoside concentration with HT-29 inhibition (r = 0.69). The concentration inhibition fifty (IC50) for BL, SH, RG on HT-29 and HCT-116 cell proliferation ranged 0.9–2.0 mg/mL. Extracts decreased expression of anti-apoptotic proteins (survivin, cIAP-2, XIAP), induced apoptosis, and arrested cells in G1. Anthocyanins exhibited tyrosine kinase inhibitory potential in silico and biochemically; cyanidin-3-O-glucoside had one of the highest binding affinities with all kinases, especially ABL1 (−8.5 kcal/mol). Cyanidin-3-O-glucoside and delphinidin-3-O-glucoside inhibited EGFR (IC50 = 0.10 and 2.37 µM, respectively). Cyanidin-3-O-glucoside was the most potent anthocyanin on kinase inhibition.

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