Comparison of real-time, quantitative PCR with molecular beacons to nested PCR and culture methods for detection of Mycobacterium avium subsp. paratuberculosis in bovine fecal samples

Y. Fang; W. H. Wu; J. L. Pepper; J. L. Larsen; S. A.E. Marras; E. A. Nelson; W. B. Epperson; J. Christopher-Hennings

doi:10.1128/JCM.40.1.287-291.2002

Comparison of real-time, quantitative PCR with molecular beacons to nested PCR and culture methods for detection of Mycobacterium avium subsp. paratuberculosis in bovine fecal samples

Y. Fang, W. H. Wu, J. L. Pepper, J. L. Larsen, S. A.E. Marras, E. A. Nelson, W. B. Epperson, J. Christopher-Hennings

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Abstract

An automated PCR with fluorescent probes (molecular beacons) detected Mycobacterium avium subsp. paratuberculosis in bovine feces. When the PCR was compared with culture in testing 41 fecal samples, kappa scores of 0.94 to 0.96, a sensitivity of 93 to 96%, and a specificity of 92% were obtained. Results were quantitated by using a standard curve derived from a plasmid containing IS900. A minimum quantity of 1.7 × 10^-4 pg of DNA, correlating to 1 to 8 CFU, was detected.

Original language	English (US)
Pages (from-to)	287-291
Number of pages	5
Journal	Journal of Clinical Microbiology
Volume	40
Issue number	1
DOIs	https://doi.org/10.1128/JCM.40.1.287-291.2002
State	Published - 2002
Externally published	Yes

ASJC Scopus subject areas

Microbiology (medical)

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Fang, Y., Wu, W. H., Pepper, J. L., Larsen, J. L., Marras, S. A. E., Nelson, E. A., Epperson, W. B., & Christopher-Hennings, J. (2002). Comparison of real-time, quantitative PCR with molecular beacons to nested PCR and culture methods for detection of Mycobacterium avium subsp. paratuberculosis in bovine fecal samples. Journal of Clinical Microbiology, 40(1), 287-291. https://doi.org/10.1128/JCM.40.1.287-291.2002

Fang, Y, Wu, WH, Pepper, JL, Larsen, JL, Marras, SAE, Nelson, EA, Epperson, WB & Christopher-Hennings, J 2002, 'Comparison of real-time, quantitative PCR with molecular beacons to nested PCR and culture methods for detection of Mycobacterium avium subsp. paratuberculosis in bovine fecal samples', Journal of Clinical Microbiology, vol. 40, no. 1, pp. 287-291. https://doi.org/10.1128/JCM.40.1.287-291.2002

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abstract = "An automated PCR with fluorescent probes (molecular beacons) detected Mycobacterium avium subsp. paratuberculosis in bovine feces. When the PCR was compared with culture in testing 41 fecal samples, kappa scores of 0.94 to 0.96, a sensitivity of 93 to 96%, and a specificity of 92% were obtained. Results were quantitated by using a standard curve derived from a plasmid containing IS900. A minimum quantity of 1.7 × 10-4 pg of DNA, correlating to 1 to 8 CFU, was detected.",

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doi = "10.1128/JCM.40.1.287-291.2002",

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AU - Epperson, W. B.

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Comparison of real-time, quantitative PCR with molecular beacons to nested PCR and culture methods for detection of Mycobacterium avium subsp. paratuberculosis in bovine fecal samples

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