Comparison of primer sets for use in automated ribosomal intergenic spacer analysis of aquatic bacterial communities: An ecological perspective

Stuart E. Jones, Ashley L. Shade, Katherine D. McMahon, Angela D. Kent

Research output: Contribution to journalArticlepeer-review

Abstract

Two primer sets for automated ribosomal intergenic spacer analysis (ARISA) were used to assess the bacterial community composition (BCC) in Lake Mendota, Wisconsin, over 3 years. Correspondence analysis revealed differences in community profiles generated by different primer sets, but overall ecological patterns were conserved in each case. ARISA is a powerful tool for evaluating BCC change through space and time, regardless of the specific primer set used.

Original languageEnglish (US)
Pages (from-to)659-662
Number of pages4
JournalApplied and environmental microbiology
Volume73
Issue number2
DOIs
StatePublished - Jan 2007

ASJC Scopus subject areas

  • Biotechnology
  • Food Science
  • Applied Microbiology and Biotechnology
  • Ecology

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