Comparación entre un micrómetro y un microscopio electrónico (MEB) en la medida del grosor de cascarones de huevos de aves

Translated title of the contribution: Comparison of micrometer- and scanning electron microscope-based measurements of avian eggshell thickness

Branislav Igic, Mark E. Hauber, Josie A. Galbraith, Tomas Grim, Donald C. Dearborn, Patricia L.R. Brennan, Csaba Moskát, Pankaj K. Choudhary, Phillip Cassey

Research output: Contribution to journalArticlepeer-review

Abstract

The study of avian eggshell structure, including composition, pigmentation, thickness, and strength, has important ecological and economic implications. Previous investigators have used a variety of techniques to derive either direct measures or indirect estimates of eggshell thickness. Assessing the repeatability and method agreement of different techniques is necessary to permit comparison of eggshell thickness values from different studies on various genetic stocks, populations, and species. We recorded and analyzed measurements of eggshell thickness using two methods, micrometers and scanning electron microscopy (SEM), for several Palaeognathae and Neognathae taxa, including nonpasserines and passerines. Applying a tolerance-interval approach, we found that repeatability of measurements for eggs with thinner shells (<300 μm, all Neognathae taxa) was worse than for eggs with thicker shells (Palaeognathae taxa), but was still statistically and biologically reasonable given that the relative magnitude of intramethod agreements was <11%. Our results support previous predictions that measurements made using a micrometer are comparable to those made using SEM. This finding is particularly important given the relative ease and cost efficiency of the micrometer method. Importantly, these new analyses can be used to validate the use of published data from previous studies of micrometer-based eggshell thickness for both intra- and interspecific comparisons.

Translated title of the contributionComparison of micrometer- and scanning electron microscope-based measurements of avian eggshell thickness
Original languageSpanish
Pages (from-to)402-410
Number of pages9
JournalJournal of Field Ornithology
Volume81
Issue number4
DOIs
StatePublished - Dec 2010
Externally publishedYes

Keywords

  • Avian eggshell
  • Electron microscope
  • Thickness measurement
  • Tolerance interval

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics

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