Comparison of hepatic microsomal drug metabolizing systems from rats fed crude and purified diets

V. Abbott, L. Deloria, T. Guenthner, E. Jeffery, A. Kotake, D. Nerland, G. Mannering

Research output: Contribution to journalArticlepeer-review

Abstract

Hepatic microsomes from rats fed a crude or a purified diet were compared by measuring their contents of protein, cytochrome P-450, and cytochrome b 5, their rates of activity of NADPH and NADH cytochrome c reductases, NADPH cytochrome P-450 reductase, NADPH oxidase, lipid peroxidase, ethylmorphine N demethylase, aniline hydroxylase, benzpyrene hydroxylase, and their substrate binding spectra (ethylmorphine, hexobarbital, aniline, and ethyl isocyanide). With the exception of lipid peroxidase activity, which was much higher in microsomes from animals fed the crude diet, little or no consistent diet related differences in these measurements were observed over a 4 wk experimental period, nor were results significantly less variable with one or the other diet. No consistent significant differences were observed with two strains of rats. The lower lipid peroxidase activity seen with the purified diet appeared to be due to the high vitamin E intake when that diet was employed; rats fed the crude diet and an oral supplement of α tocopherol yielded microsomes with low lipid peroxidase activities similar to those seen in microsomes from rats fed the purified diet. A gradual temporal increase in benzpyrene hydroxylase activity was observed with both diets. This was interpreted to be due to environmental inducing agents other than those present in the diet.

Original languageEnglish (US)
Pages (from-to)215-222
Number of pages8
JournalDrug Metabolism and Disposition
Volume4
Issue number3
StatePublished - 1976
Externally publishedYes

ASJC Scopus subject areas

  • Pharmacology
  • Pharmaceutical Science

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