TY - JOUR
T1 - Comparison of equine tendon-and bone marrow-derived cells cultured on tendon matrix with or without insulin-like growth factor-I supplementation
AU - Durgam, Sushmitha S.
AU - Stewart, Allison A.
AU - Pondenis, Holly C.
AU - Gutierrez-Nibeyro, Santiago M.
AU - Evans, Richard B.
AU - Stewart, Matthew C.
PY - 2012/1
Y1 - 2012/1
N2 - Objective-To compare in vitro expansion, explant colonization, and matrix synthesis of equine tendon-and bone marrow-derived cells in response to insulin-like growth factor-I (IGF-I) supplementation. Sample-Cells isolated from 7 young adult horses. Procedures-Tendon-and bone marrow-derived progenitor cells were isolated, evaluated for yield, and cultured on autogenous cell-free tendon matrix for 7 days. Samples were analyzed for cell viability and expression of collagen type I, collagen type III, and cartilage oligomeric matrix protein mRNAs. Collagen and glycosaminoglycan syntheses were quantified over a 24-hour period. Results-Tendon-and bone marrow-derived cells required 17 to 19 days of monolayer culture to reach 2 passages. Mean ± SE number of monolayer cells isolated was higher for tendon-derived cells (7.9 ± 0.9 X 10 6) than for bone marrow-derived cells (1.2 ± 0.1 X 10 6). Cell numbers after culture for 7 days on acellular tendon matrix were 1.6-to 2.8-fold higher for tendon-derived cells than for bone marrow-derived cells and 0.8-to 1.7-fold higher for IGF-I supplementation than for untreated cells. New collagen and glycosaminoglycan syntheses were significantly greater in tendon-derived cell groups and in IGF-I-supplemented groups. The mRNA concentrations of collagen type I, collagen type III, and cartilage oligomeric matrix protein were not significantly different between tendon-and bone marrow-derived groups. Conclusions and Clinical Relevance-In vitro results of this study suggested that tendonderived cells supplemented with IGF-I may offer a useful resource for cell-based strategies in tendon healing.
AB - Objective-To compare in vitro expansion, explant colonization, and matrix synthesis of equine tendon-and bone marrow-derived cells in response to insulin-like growth factor-I (IGF-I) supplementation. Sample-Cells isolated from 7 young adult horses. Procedures-Tendon-and bone marrow-derived progenitor cells were isolated, evaluated for yield, and cultured on autogenous cell-free tendon matrix for 7 days. Samples were analyzed for cell viability and expression of collagen type I, collagen type III, and cartilage oligomeric matrix protein mRNAs. Collagen and glycosaminoglycan syntheses were quantified over a 24-hour period. Results-Tendon-and bone marrow-derived cells required 17 to 19 days of monolayer culture to reach 2 passages. Mean ± SE number of monolayer cells isolated was higher for tendon-derived cells (7.9 ± 0.9 X 10 6) than for bone marrow-derived cells (1.2 ± 0.1 X 10 6). Cell numbers after culture for 7 days on acellular tendon matrix were 1.6-to 2.8-fold higher for tendon-derived cells than for bone marrow-derived cells and 0.8-to 1.7-fold higher for IGF-I supplementation than for untreated cells. New collagen and glycosaminoglycan syntheses were significantly greater in tendon-derived cell groups and in IGF-I-supplemented groups. The mRNA concentrations of collagen type I, collagen type III, and cartilage oligomeric matrix protein were not significantly different between tendon-and bone marrow-derived groups. Conclusions and Clinical Relevance-In vitro results of this study suggested that tendonderived cells supplemented with IGF-I may offer a useful resource for cell-based strategies in tendon healing.
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U2 - 10.2460/ajvr.73.1.153
DO - 10.2460/ajvr.73.1.153
M3 - Article
C2 - 22204302
AN - SCOPUS:84855290551
SN - 0002-9645
VL - 73
SP - 153
EP - 161
JO - American journal of veterinary research
JF - American journal of veterinary research
IS - 1
ER -