Comparison of cushioned centrifugation and SpermFilter filtration on longevity and morphology of cooled-stored equine semen

J. Roach, M. Schnobrich, R. Ellerbrock, L. Feijo, E. Bradecamp, M. A. Alvarenga, K. Kline, I. Canisso

Research output: Contribution to journalArticlepeer-review

Abstract

This study compares two methods for seminal plasma removal by evaluating sperm recovery rates, and motility and morphology of cooled-stored semen. Ejaculates were divided into three groups: control, filtration and cushioned centrifugation. Semen was extended to 25 million sperm/ml using a skim-milk-based extender and stored at 5°C for all groups. Sperm motility (total motility (%TM) and progressive motility (%PM)) was determined at 0, 24, 48 and 72 hours by a computer-Assisted sperm analyser. Sperm morphology was assessed using differential interference microscopy. Overall, %TM of the centrifugation group was significantly higher than the filter group, but not significantly different than the control. No significant difference in %TM or %PM was detected for the control group and filter. Cushioned centrifugation was a superior method to obtain progressively motile sperm compared with control (P=0.03) and filter groups (P<0.001). No significant difference was found for the per cent of normal sperm cells and detached heads between the groups. This study demonstrated that cushioned centrifugation was a superior method to remove seminal plasma while preserving %TM and enhancing %PM for stallions under cooled storage over three days. However, as the differences appear to be negligible, the SpermFilter may represent an alternative for farms lacking a centrifuge.

Original languageEnglish (US)
Pages (from-to)241
Number of pages1
JournalVeterinary Record
Volume178
Issue number10
DOIs
StatePublished - Mar 5 2016

ASJC Scopus subject areas

  • veterinary(all)

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