TY - JOUR
T1 - Comparison of breast milk vitamin A concentration measured in fresh milk by a rapid field assay (the iCheck FLUORO) with standard measurement of stored milk by HPLC
AU - Engle-Stone, Reina
AU - Haskell, Marjorie
AU - La Frano, Michael R.
AU - Ndjebayi, Alex Ongla
AU - Nankap, Martin
AU - Brown, Kenneth
N1 - Funding Information:
We thank Daniela Hampel (USDA, Davis, CA, USA) for NIST formula verification. This study was supported by Sight and Life (Basel, Switzerland).
PY - 2014/8
Y1 - 2014/8
N2 - Availability of rapid, point-of-contact analytical methods would facilitate the use of breast milk vitamin A concentration (BMVA) to assess vitamin A (VA) status. We compared BMVA concentrations measured by high-performance liquid chromatography (HPLC) (the standard technique) with those by iCheck FLUORO, a new portable fluorometer that can rapidly quantify BMVA. Casual breast milk samples (n=154) were collected during a representative survey in Yaoundé and Douala, Cameroon. Milk fat and BMVA concentrations (by iCheck) were measured in fresh milk in the field. After storage at <-20 °C, BMVA concentrations were also measured by HPLC. BMVA values from the two methods were highly correlated (R 2 =0.72 for BMVA/l; R 2 =0.62 for BMVA/g fat, both P<0.0001). HPLC values were greater than iCheck values on average, and the difference increased with increasing BMVA. The iCheck FLUORO could be useful for monitoring fortification programs, but before-after surveys to assess change in BMVA concentrations should use one method consistently.
AB - Availability of rapid, point-of-contact analytical methods would facilitate the use of breast milk vitamin A concentration (BMVA) to assess vitamin A (VA) status. We compared BMVA concentrations measured by high-performance liquid chromatography (HPLC) (the standard technique) with those by iCheck FLUORO, a new portable fluorometer that can rapidly quantify BMVA. Casual breast milk samples (n=154) were collected during a representative survey in Yaoundé and Douala, Cameroon. Milk fat and BMVA concentrations (by iCheck) were measured in fresh milk in the field. After storage at <-20 °C, BMVA concentrations were also measured by HPLC. BMVA values from the two methods were highly correlated (R 2 =0.72 for BMVA/l; R 2 =0.62 for BMVA/g fat, both P<0.0001). HPLC values were greater than iCheck values on average, and the difference increased with increasing BMVA. The iCheck FLUORO could be useful for monitoring fortification programs, but before-after surveys to assess change in BMVA concentrations should use one method consistently.
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U2 - 10.1038/ejcn.2014.63
DO - 10.1038/ejcn.2014.63
M3 - Article
C2 - 24736678
AN - SCOPUS:84905859979
SN - 0954-3007
VL - 68
SP - 938
EP - 940
JO - European Journal of Clinical Nutrition
JF - European Journal of Clinical Nutrition
IS - 8
ER -