Comparative pathology in ferrets infected with H1N1 influenza A viruses isolated from different hosts

Jennifer Humberd Smith, Tamas Nagy, Elizabeth Driskell, Paula Brooks, S. Mark Tompkins, Ralph A. Tripp

Research output: Contribution to journalArticlepeer-review


Virus replication and pulmonary disease pathogenesis in ferrets following intranasal infection with a pandemic influenza virus strain (A/California/4/09 [CA09]), a human seasonal influenza H1N1 virus isolate (A/New Caledonia/20/99 [Ncal99]), a classical swine influenza H1N1 virus isolate (A/Swine/Iowa/15/30 [Sw30]), or an avian H1N1 virus isolate (A/Mallard/MN/A108-2355/08 [Mal08]) were compared. Nasal wash virus titers were similar for Ncal99 and Sw30, with peak virus titers of 10 5.1 50% tissue culture infectious doses (TCID 50)/ml and 10 5.5 TCID 50/ml occurring at day 3 postinfection (p.i.), respectively. The mean peak titer for CA09 also occurred at day 3 p.i. but was higher (10 7 TCID 50/ml). In contrast, the peak virus titers (103.6 to 104.3 TCID 50/ml) for Mal08 were delayed, occurring between days 5 and 7 p.i. Disease pathogenesis was characterized by microscopic lesions in the nasal turbinates and lungs of all ferrets; however, Sw30 infection was associated with severe bronchointerstitial pneumonia. The results demonstrate that although CA09 is highly transmissible in the human population and replicates well in the ferret model, it causes modest disease compared to other H1N1 viruses, particularly Sw30 infection.

Original languageEnglish (US)
Pages (from-to)7572-7581
Number of pages10
JournalJournal of virology
Issue number15
StatePublished - Aug 2011
Externally publishedYes

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology


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