Comparative genotoxicity of nitrosamine drinking water disinfection byproducts in Salmonella and mammalian cells

Elizabeth D. Wagner, Kang Mei Hsu, Angelica Lagunas, William A. Mitch, Michael J. Plewa

Research output: Contribution to journalArticlepeer-review

Abstract

Nitrosamine water disinfection byproducts (DBPs) are an emerging class of non-halogenated, nitrogen-containing water contaminants. Five nitrosamine DBPs were analyzed for genotoxicity (N-nitrosodimethylamine (NDMA), N-nitrosopiperidine (NPIP), N-nitrosopyrrolidine (NPYR), N-nitrosomorpholine (NMOR) and N-nitrosodiphenylamine (NDPhA). Using Salmonella typhimurium strain YG7108 the descending rank order of mutagenicity was NDMA > NPIP > NMOR > NPYR; NDPhA was not mutagenic. We developed and calibrated an exogenous S9 mix that was highly effective in activating NDMA in Chinese hamster ovary (CHO) cells using the SCGE (Comet) assay. The descending rank order for genotoxicity was NDMA > NPIP > NMOR. NDPhA was genotoxic only at one concentration and NPYR was not genotoxic. The genotoxic potencies in S. typhimurium and CHO cells were highly correlated. Based on their comparative genotoxicity attention should be focused on the generation and occurrence of NDMA, NPIP and NMOR. Current drinking water disinfection processes may need to be modified such that the generation of nitrosamine DBPs is effectively limited in order to protect the environment and the public health.

Original languageEnglish (US)
Pages (from-to)109-115
Number of pages7
JournalMutation Research - Genetic Toxicology and Environmental Mutagenesis
Volume741
Issue number1-2
DOIs
StatePublished - Jan 24 2012

Keywords

  • CHO cells
  • Comet
  • Disinfection byproducts
  • S9 mix
  • SCGE
  • Salmonella
  • YG7108

ASJC Scopus subject areas

  • Genetics
  • Health, Toxicology and Mutagenesis

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