Comparative analysis of the RcsC sensor kinase from Erwinia amylovora and other enterobacteria

D. P. Wang, Youfu Zhao, S. S. Korban

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We have previously demonstrated that, though the Erwinia amylovora rcsC mutant produces more amylovoran than the Ea1189 wild type (WT) strain in vitro, it is avirulent on host plants. In this study, we further characterized the RcsC and its homologs from various enterobacteria. Complementation studies showed that RcsC homologs from Pantoea stewartii, Salmonella enterica and Yersinia pestis, but not that from E. coli, partially restored virulence of the Erwinia rcsC mutant on 'Gala' apple shoots. However, all RcsC homologs could not rescue amylovoran production phenotype of the Erwinia rcsC mutant in vitro. In addition, a chimeric construct containing the sensor domain of Erwinia RcsC and the output domain of E. coli RcsC restored virulence of the Erwinia rcsC mutant, but not amylovoran production; whereas a chimeric construct containing the sensor domain of E. coli RcsC and the output domain of Erwinia RcsC rescued amylovoran production phenotype of the Erwinia rcsC mutant, but not virulence. These results suggest that the sensor domain of RcsC might be important for regulating bacterial virulence, whereas the output domain of RcsC might be responsible for amylovoran production in vitro.

Original languageEnglish (US)
Title of host publicationXII International Workshop on Fire Blight
EditorsP. Sobiczewski, J. Pulawska, M. Kaluzna
Pages165-172
Number of pages8
StatePublished - May 13 2011

Publication series

NameActa Horticulturae
Volume896
ISSN (Print)0567-7572

Fingerprint

Erwinia
Erwinia amylovora
phosphotransferases (kinases)
virulence
mutants
Escherichia coli
Pantoea stewartii
Yersinia pestis
phenotype
Salmonella enterica
host plants
apples
shoots

Keywords

  • Chimeric protein
  • Kinase
  • Rcs phosphorelay
  • Two-component system
  • Virulence

ASJC Scopus subject areas

  • Horticulture

Cite this

Wang, D. P., Zhao, Y., & Korban, S. S. (2011). Comparative analysis of the RcsC sensor kinase from Erwinia amylovora and other enterobacteria. In P. Sobiczewski, J. Pulawska, & M. Kaluzna (Eds.), XII International Workshop on Fire Blight (pp. 165-172). (Acta Horticulturae; Vol. 896).

Comparative analysis of the RcsC sensor kinase from Erwinia amylovora and other enterobacteria. / Wang, D. P.; Zhao, Youfu; Korban, S. S.

XII International Workshop on Fire Blight. ed. / P. Sobiczewski; J. Pulawska; M. Kaluzna. 2011. p. 165-172 (Acta Horticulturae; Vol. 896).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Wang, DP, Zhao, Y & Korban, SS 2011, Comparative analysis of the RcsC sensor kinase from Erwinia amylovora and other enterobacteria. in P Sobiczewski, J Pulawska & M Kaluzna (eds), XII International Workshop on Fire Blight. Acta Horticulturae, vol. 896, pp. 165-172.
Wang DP, Zhao Y, Korban SS. Comparative analysis of the RcsC sensor kinase from Erwinia amylovora and other enterobacteria. In Sobiczewski P, Pulawska J, Kaluzna M, editors, XII International Workshop on Fire Blight. 2011. p. 165-172. (Acta Horticulturae).
Wang, D. P. ; Zhao, Youfu ; Korban, S. S. / Comparative analysis of the RcsC sensor kinase from Erwinia amylovora and other enterobacteria. XII International Workshop on Fire Blight. editor / P. Sobiczewski ; J. Pulawska ; M. Kaluzna. 2011. pp. 165-172 (Acta Horticulturae).
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