Abstract
We have previously demonstrated that, though the Erwinia amylovora rcsC mutant produces more amylovoran than the Ea1189 wild type (WT) strain in vitro, it is avirulent on host plants. In this study, we further characterized the RcsC and its homologs from various enterobacteria. Complementation studies showed that RcsC homologs from Pantoea stewartii, Salmonella enterica and Yersinia pestis, but not that from E. coli, partially restored virulence of the Erwinia rcsC mutant on 'Gala' apple shoots. However, all RcsC homologs could not rescue amylovoran production phenotype of the Erwinia rcsC mutant in vitro. In addition, a chimeric construct containing the sensor domain of Erwinia RcsC and the output domain of E. coli RcsC restored virulence of the Erwinia rcsC mutant, but not amylovoran production; whereas a chimeric construct containing the sensor domain of E. coli RcsC and the output domain of Erwinia RcsC rescued amylovoran production phenotype of the Erwinia rcsC mutant, but not virulence. These results suggest that the sensor domain of RcsC might be important for regulating bacterial virulence, whereas the output domain of RcsC might be responsible for amylovoran production in vitro.
| Original language | English (US) |
|---|---|
| Title of host publication | XII International Workshop on Fire Blight |
| Editors | P. Sobiczewski, J. Pulawska, M. Kaluzna |
| Pages | 165-172 |
| Number of pages | 8 |
| State | Published - May 13 2011 |
Publication series
| Name | Acta Horticulturae |
|---|---|
| Volume | 896 |
| ISSN (Print) | 0567-7572 |
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Keywords
- Chimeric protein
- Kinase
- Rcs phosphorelay
- Two-component system
- Virulence
ASJC Scopus subject areas
- Horticulture
Cite this
Comparative analysis of the RcsC sensor kinase from Erwinia amylovora and other enterobacteria. / Wang, D. P.; Zhao, Youfu; Korban, S. S.
XII International Workshop on Fire Blight. ed. / P. Sobiczewski; J. Pulawska; M. Kaluzna. 2011. p. 165-172 (Acta Horticulturae; Vol. 896).Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
}
TY - GEN
T1 - Comparative analysis of the RcsC sensor kinase from Erwinia amylovora and other enterobacteria
AU - Wang, D. P.
AU - Zhao, Youfu
AU - Korban, S. S.
PY - 2011/5/13
Y1 - 2011/5/13
N2 - We have previously demonstrated that, though the Erwinia amylovora rcsC mutant produces more amylovoran than the Ea1189 wild type (WT) strain in vitro, it is avirulent on host plants. In this study, we further characterized the RcsC and its homologs from various enterobacteria. Complementation studies showed that RcsC homologs from Pantoea stewartii, Salmonella enterica and Yersinia pestis, but not that from E. coli, partially restored virulence of the Erwinia rcsC mutant on 'Gala' apple shoots. However, all RcsC homologs could not rescue amylovoran production phenotype of the Erwinia rcsC mutant in vitro. In addition, a chimeric construct containing the sensor domain of Erwinia RcsC and the output domain of E. coli RcsC restored virulence of the Erwinia rcsC mutant, but not amylovoran production; whereas a chimeric construct containing the sensor domain of E. coli RcsC and the output domain of Erwinia RcsC rescued amylovoran production phenotype of the Erwinia rcsC mutant, but not virulence. These results suggest that the sensor domain of RcsC might be important for regulating bacterial virulence, whereas the output domain of RcsC might be responsible for amylovoran production in vitro.
AB - We have previously demonstrated that, though the Erwinia amylovora rcsC mutant produces more amylovoran than the Ea1189 wild type (WT) strain in vitro, it is avirulent on host plants. In this study, we further characterized the RcsC and its homologs from various enterobacteria. Complementation studies showed that RcsC homologs from Pantoea stewartii, Salmonella enterica and Yersinia pestis, but not that from E. coli, partially restored virulence of the Erwinia rcsC mutant on 'Gala' apple shoots. However, all RcsC homologs could not rescue amylovoran production phenotype of the Erwinia rcsC mutant in vitro. In addition, a chimeric construct containing the sensor domain of Erwinia RcsC and the output domain of E. coli RcsC restored virulence of the Erwinia rcsC mutant, but not amylovoran production; whereas a chimeric construct containing the sensor domain of E. coli RcsC and the output domain of Erwinia RcsC rescued amylovoran production phenotype of the Erwinia rcsC mutant, but not virulence. These results suggest that the sensor domain of RcsC might be important for regulating bacterial virulence, whereas the output domain of RcsC might be responsible for amylovoran production in vitro.
KW - Chimeric protein
KW - Kinase
KW - Rcs phosphorelay
KW - Two-component system
KW - Virulence
UR - http://www.scopus.com/inward/record.url?scp=80053357079&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=80053357079&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:80053357079
SN - 9789066051171
T3 - Acta Horticulturae
SP - 165
EP - 172
BT - XII International Workshop on Fire Blight
A2 - Sobiczewski, P.
A2 - Pulawska, J.
A2 - Kaluzna, M.
ER -