Compact quantum dots for quantitative cytology

Phuong Le, Shweta Chitoor, Chunlai Tu, Sung Jun Lim, Andrew M. Smith

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

In this chapter, we describe the preparation of fluorescent quantum dots for imaging and measuring protein expression in cells. Quantum dots are nanocrystals that have numerous advantages for biomolecular detection compared with organic dyes and fluorescent proteins, but their large size has been a limiting factor. We describe the synthesis of nanoparticles smaller than 10 nm (smaller than an antibody), their attachment to monoclonal antibodies through click chemistry, characterization of the conjugates, and use for labeling of cellular antigens. We further discuss the unique advantages and challenges associated with this approach compared with conventional immunofluorescence techniques.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages147-158
Number of pages12
DOIs
StatePublished - Jan 1 2020

Publication series

NameMethods in Molecular Biology
Volume2064
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Antibody
  • Cancer
  • Cytology
  • EGFR
  • Fluorescence
  • Imaging
  • Immunofluorescence
  • Microscopy
  • Nanocrystal
  • Nanotechnology
  • Protein
  • Single cell

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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  • Cite this

    Le, P., Chitoor, S., Tu, C., Lim, S. J., & Smith, A. M. (2020). Compact quantum dots for quantitative cytology. In Methods in Molecular Biology (pp. 147-158). (Methods in Molecular Biology; Vol. 2064). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-9831-9_12