Compact quantum dots for quantitative cytology

Phuong Le, Shweta Chitoor, Chunlai Tu, Sung Jun Lim, Andrew M Smith

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

In this chapter, we describe the preparation of fluorescent quantum dots for imaging and measuring protein expression in cells. Quantum dots are nanocrystals that have numerous advantages for biomolecular detection compared with organic dyes and fluorescent proteins, but their large size has been a limiting factor. We describe the synthesis of nanoparticles smaller than 10 nm (smaller than an antibody), their attachment to monoclonal antibodies through click chemistry, characterization of the conjugates, and use for labeling of cellular antigens. We further discuss the unique advantages and challenges associated with this approach compared with conventional immunofluorescence techniques.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages147-158
Number of pages12
DOIs
StatePublished - Jan 1 2020

Publication series

NameMethods in Molecular Biology
Volume2064
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Fingerprint

Quantum Dots
Nanoparticles
Cell Biology
Click Chemistry
Fluorescent Dyes
Fluorescent Antibody Technique
Proteins
Monoclonal Antibodies
Antigens
Antibodies

Keywords

  • Antibody
  • Cancer
  • Cytology
  • EGFR
  • Fluorescence
  • Imaging
  • Immunofluorescence
  • Microscopy
  • Nanocrystal
  • Nanotechnology
  • Protein
  • Single cell

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Le, P., Chitoor, S., Tu, C., Lim, S. J., & Smith, A. M. (2020). Compact quantum dots for quantitative cytology. In Methods in Molecular Biology (pp. 147-158). (Methods in Molecular Biology; Vol. 2064). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-9831-9_12

Compact quantum dots for quantitative cytology. / Le, Phuong; Chitoor, Shweta; Tu, Chunlai; Lim, Sung Jun; Smith, Andrew M.

Methods in Molecular Biology. Humana Press Inc., 2020. p. 147-158 (Methods in Molecular Biology; Vol. 2064).

Research output: Chapter in Book/Report/Conference proceedingChapter

Le, P, Chitoor, S, Tu, C, Lim, SJ & Smith, AM 2020, Compact quantum dots for quantitative cytology. in Methods in Molecular Biology. Methods in Molecular Biology, vol. 2064, Humana Press Inc., pp. 147-158. https://doi.org/10.1007/978-1-4939-9831-9_12
Le P, Chitoor S, Tu C, Lim SJ, Smith AM. Compact quantum dots for quantitative cytology. In Methods in Molecular Biology. Humana Press Inc. 2020. p. 147-158. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-9831-9_12
Le, Phuong ; Chitoor, Shweta ; Tu, Chunlai ; Lim, Sung Jun ; Smith, Andrew M. / Compact quantum dots for quantitative cytology. Methods in Molecular Biology. Humana Press Inc., 2020. pp. 147-158 (Methods in Molecular Biology).
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