Combined fluorescent and gold nucleic acid probes

Richard D. Powell, James F. Hainfeld, Mair E.A. Churchill, Andrew S Belmont

Research output: Contribution to conferencePaperpeer-review

Abstract

Two approaches were used to synthesize site specific probes which incorporate both fluorophores and the 1.4 nm Nanogold gold particle. One method involves the covalent linkage of the Nanogold particle to Hoescht-33258, a fluorescent dye which is specific for AT-rich regions of double stranded DNA. In the second approach, a label was prepared which consists of a small number of fluorescein groups and the Nanogold particle. Labeled peptides were separated from unbound peptide by gel filtration.

Original languageEnglish (US)
Pages176-177
Number of pages2
StatePublished - Dec 1 1994
Externally publishedYes
EventProceedings of the 52nd Annual Meeting of the Microscopy Society of America - New Orleans, LA, USA
Duration: Jul 31 1994Aug 5 1994

Other

OtherProceedings of the 52nd Annual Meeting of the Microscopy Society of America
CityNew Orleans, LA, USA
Period7/31/948/5/94

ASJC Scopus subject areas

  • Engineering(all)

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