@inbook{9d4995e65af6426e89165c57e91d7389,
title = "Combined and iterative use of computational design and directed evolution for protein-ligand binding design",
abstract = "The advantages of computational design and directed evolution are complementary, and only through combined and iterative use of both approaches, a daunting task such as protein-ligand interaction design, can be achieved efficiently. Here, we describe a systematic strategy to combine structure-guided computational design, iterative site saturation mutagenesis, and yeast two-hybrid system (Y2H)-based phenotypic screening to engineer novel and orthogonal interactions between synthetic ligands and human estrogen receptor α (hERα) for the development of novel gene switches.",
keywords = "Computational design, Directed evolution, Gene switch, Protein-ligand interaction",
author = "Meng Wang and Huimin Zhao",
note = "Publisher Copyright: {\textcopyright} Springer Science+Business Media New York 2016.",
year = "2016",
month = may,
day = "1",
doi = "10.1007/978-1-4939-3569-7_8",
language = "English (US)",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "139--153",
booktitle = "Methods in Molecular Biology",
}