Combined and iterative use of computational design and directed evolution for protein-ligand binding design

Meng Wang, Huimin Zhao

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

The advantages of computational design and directed evolution are complementary, and only through combined and iterative use of both approaches, a daunting task such as protein-ligand interaction design, can be achieved efficiently. Here, we describe a systematic strategy to combine structure-guided computational design, iterative site saturation mutagenesis, and yeast two-hybrid system (Y2H)-based phenotypic screening to engineer novel and orthogonal interactions between synthetic ligands and human estrogen receptor α (hERα) for the development of novel gene switches.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages139-153
Number of pages15
DOIs
StatePublished - May 1 2016

Publication series

NameMethods in Molecular Biology
Volume1414
ISSN (Print)1064-3745

Keywords

  • Computational design
  • Directed evolution
  • Gene switch
  • Protein-ligand interaction

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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  • Cite this

    Wang, M., & Zhao, H. (2016). Combined and iterative use of computational design and directed evolution for protein-ligand binding design. In Methods in Molecular Biology (pp. 139-153). (Methods in Molecular Biology; Vol. 1414). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-3569-7_8