Combinatorial approach for fabrication of coatings to control bacterial adhesion

S. Pedron, C. Peinado, F. Catalina, P. Bosch, K. S. Anseth, C. Abrusci

Research output: Contribution to journalArticlepeer-review


Due to the high importance of bacterial infections in medical devices there is an increasing interest in the design of anti-fouling coatings. The application of substrates with controlled chemical gradients to prevent microbial adhesion is presented. We describe here the co-polymerization of poly(ethylene glycol) dimethacrylate with a hyperbranched multimethacrylate (H30MA) using a chemical gradient generator; and the resulting films were characterized with respect to their ability to serve as coating for biomedical devices. The photo-polymerized materials present special surface properties due to the hyperbranched structure of H30MA and phase separation at specific concentrations in the PEGDM matrix. This approach affords the investigation of cell response to a large range of different chemistries on a single sample. Two bacterial strains commonly associated with surgical site infections, Escherichia coli and Pseudomonas aeruginosa, have been cultured on these substrates to study their attachment behaviour. These gradient-coated samples demonstrate less bacterial adhesion at higher concentrations of H30MA, and the adhesion is substantially affected by the extent of surface phase segregation.

Original languageEnglish (US)
Pages (from-to)1613-1628
Number of pages16
JournalJournal of Biomaterials Science, Polymer Edition
Issue number12
StatePublished - 2012


  • Anti-fouling
  • Bacterial adhesion
  • Cross-linking gradient
  • Hyperbranched polymers
  • Microfluidics
  • Surface gradients

ASJC Scopus subject areas

  • Biophysics
  • Bioengineering
  • Biomaterials
  • Biomedical Engineering


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